Leaves extracts of Gongronema Latifolium and Ocimum Gratissimum offer synergy on organ weights alleviation and pancreatic resurgence against Streptozotocin diabetic rats
DOI:
https://doi.org/10.56511/JIPBS.2015.v2.i04.pp522-536Abstract
This study unveiled the pancreatic regenerative capacity and organs weight alleviation of combined leaves extracts of Gongronema latifolium (GL) and Ocimum gratissimum (OG) against streptozotocin-induced diabetic rats. Thirty-six male albino rats were divided into 6 groups of 6 rats each. Groups A and F received placebo treatment and served as diabetic and normal controls respectively, B, C and D respectively received 200mg/kg b.w GL and OG and 100mg/kg b.w. each of combined GLOG. and E received 5 IU/kg b.w insulin (subcutaneously). During 28 days of treatment, daily changes in blood glucose were measured. After this period, animals were sacrificed and organs viz: liver, pancreas, kidney and heart collected and weighed. Serum was collected for alpha-amylase analysis. From the results, the extracts significantly (p<0.05) attenuated STZ elevation in percentage blood glucose concentrations by decreasing their levels. There was significant (P<0.05) increase in relative organ weights of diabetic control rats compared to normal control and treated rats. The combined extracts treated animals exhibited significant (P<0.05) decrease in relative organ weights as compared to single extracts treated groups. The activity of serum alpha amylase decreased (380.08±0.34) significantly in diabetic control when compared to normal control and all treatment groups. In combined extracts treated rats, its activity decreased (438.44±0.47) significantly (p<0.05) when compared to single extracts treated rats, hence displaying synergy. The histology of pancreas of extract treated animals reversed the pancreatic lesions observed in diabetic control rats. Comparatively, the combined extracts displayed a significant synergistic pancreatic resuscitating effect than single extracts.
Keywords:
Gongronema latifolium, Ocimum gratissimum, Streptozotocin, Pancreas, diabetes, ratsDownloads
References
Mahsud, MA J, Khan A, Hussain J. Hematological changes in tobacco using type 2 diabetic patients. Gomal. J Med Sci. 2010; 8:8-11.
King H, Aubert RE, Herman WH. Global burden of diabetes, 1995-2025: prevalence, numerical estimates, and projections. Diabetes Care, 1998; 21:141431.
Wild S, Roglic G, Green A, Sicree R, King H: Global prevalence of diabetes: estimates for the year 2000 and projections for 2030. Diabetes Care 2004, 27:1047 – 1053.
Mathis D, Vence L, Benoist C: Beta-Cell death during progression to diabetes. Nature 2001, 414:792 – 798.
Pitkänen OM, Martin JM, Hallman M, Åkerblom HK, Sariola H, Andersson SM. Free radical activity during development of insulin-dependent diabetes mellitus in the rat. Life Sci.; 1992, 50 (5):335 – 339.
Thulesen, J., Orskov, C., Hoist, J.J. & Paulsen, S.S. Short-term insulin treatment prevents the diabetogenic anction of streptozotocin in rats. Endocrinology, 1997, 138: 62-68.
Rodrigues, B., Poucheret, P., Betell, M.L. & Mc Neill, J.N.. Streptozotocin induced Diabetes: Induction, Mechanism(s), and Dose Dep endency. In J. H. McNeill’s (Ed.), Experimental Models of Diabetes, 1999, pp. 3-17.
Elsner, M., Guldkakke, B., Tiedge, M., Mu iday, R. & Lenzen, S. Relative importance of transport and alkylation for pancreatic bete-cell toxicity of streptozotocin. Diabetologia, 2000, 43: I528-I 533.
Turk, J., Corbett, J. A., Ramanadham, S., Bohrer, A. & Mcdaniel, M.L. Biochemical Evidence For Nitric Oxide formation from streptozotocin in isolated pancreatic islets. Biochemical, Biophysics Research Communications, 1993, 97: 1458-1464.
Szkudelski T. The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiol. Res. 2001, 50(6): 537-46.
Larsen JL: Pancreas transplantation: indications and consequences. Endocr Rev 2004, 25:919 – 946.
Shapiro AM, Lakey JR, Ryan EA, Korbutt GS, Toth E, Warnock GL, Kneteman NM, Rajotte RV: Islet transplantation in seven patients with type 1 diabetes mellitus using a glucocorticoid free immunosuppre ssive regimen. N Eng J Med 2000, 343:230 – 238.
Ryan EA, Lakey JR, Rajotte RV, Korbutt GS, Kin T, Imes S, Rabinovitch A, Elliott JF, Bigam D, Kneteman NM, Warnock GL, Larsen I, Shapiro AM: Clinical outcomes and insulin secretion after islet transplantation with the Edmonton protocol. Diabetes 2001, 50:710 – 719.
Yamaoka T: Regeneration therapy of pancreatic beta cells: towards a cure for diabetes? Biochem Biophys Res Commun 2002, 296:1039 – 1043.
Gruessner, A.C. & Sutherland, D.E. Pancreas transplant outcomes for United States (US) and non-US cases as reported to United Network for Organ Sharing (UNOS) and the Internatio nal Pancreas Transplant Registry (IPTR). Clinical Transplantation, 2002, 41: 34-39.
Sutherland, D.E., Gruessner, A. & Herin, B.J. Beta-cell replacement therapy (pancrease and islet transplantation) for the treatment of Diabetes Mellitus: an integrated approach. Endocrinology and Metabolism, 2004, 33: 135-148.
Spellman, C.W. Islet cell dysfunction in progress to diabetes mellitus. Journal of American Osteopathology Association, 2007, p. 36.
Meiver-Levy, I. & Ferber, S. New organs from our own tissues: Liver-to-pancrease transdifferentiation. Trends in Endocrino logy and Metabolism 2004, 14: 460-466.
Robertson, R.P. Pancreas transplantation for patients with Type 1 diabetes. Diabetes Care, 2004, 27 (1): 5105.
Matsumoto S, Noguchi H, Hatanaka N, Shimoda M, Kobayashi N, Jackson A, Onaca N, Naziruddin B, Levy MF: Estimation of donor usability for islet transplantation in the United States with the kyoto islet isolation method. Cell Transplant 2009, 18:549 – 556.
Yaro AH, Anuka JA, Salawu OA, Magaji MG. Anticonvulsant activities of methanol extract of Chrysanthellum indicum linn. Vatke in mice and chicks. Niger. J. Pharm. Sci., 2007, 6(2): 22-27
Risbud MV, Bhonde RR: Models of pancreatic regeneration in diabetes. Diab Res Clin Pract 2002, 58:155 – 165.
Vague P, Picq R, Bernal M, LassmannVague V, Vialettes B: Effect of nicotinamide treatment on the residual insulin secretion in type 1 (insulin dependant) diabetic patients. Diabetologia 1989, 32:316 – 321.
Bolkent S, Yanardag R, Tabakoglu-Oguz A, Ozsoy-Sacan O: Effects of Chard extract (Beta vilgaris L. var. Cicla) extract on pancreatic beta-cells in streptozotocin – diabetic rats: a morphological and biochemical study.J Ethanopharmacol 2000, 73:251 – 259.
Xiu LM, Miura AB, Yamamoto K, Kobayashi T, Song QH, Kitamura H, Cyong JC: Pancreatic islet regeneration by ephedrine in mice with streptozotocin-induced diabetes. Am J Chin Med, 2001, 29:493 – 500.
Okafor, J.C. Identification and conservation of plants used in traditional medicine. Lead lecture presented at the international workshop on evaluation of traditional medicine. University of Nigeria, Nsukka, 1987.
Morebise, O., Fafunso, M.A., Makinde, J. M., Olayide, O. A. & Awe, E. Anti-inflammatory Property of Gongronema latifolium. Phytotherapy Research, 2002, 16: 575577.
Farombi, E.O. African indigenous plants with chemotherapeutic potentials and sbiotechnological approach to the production of bio active prophylactic agents. African Journal of biotechnology, 2003, 2(12): 662-671.
Antai, A.B., Ofem, O.E., Ikpi, D.E., Ukafia, S. & Agiang, E. A phytochemistry and some haematological changes following oral administration of ethanolic root extract of gongronema latifolium in rats. Nigerian Journal of Physiological Sciences, 2009 24(1): 79 – 8.
Iweala, E.J. & Obidoa, O. Some biochemical, haematological and histological responses to a long term of a diet supplemented with leaves of Gongronema latifolium in male albino wistar rat. Journal of Biological Sciences, 2009, 9(8): 859-865.
Sulistiarini, D.L. Ocimum gratissimum L. In: Oyen PA, Nguyen ED (eds) Plant Resources of South-East Asia. No. 19: Essential oils Plants (Prosea Foundation, Bogor, Indonesia) 1999, p. 140
Sofowora, L.A. Medicinal plants and traditional medicine in Africa. Spectrum Books Ltd, Ibadan, Nigeria. Nigeria. 1993, p. 55.
Leal, P.F., Chaves, F., Celio, M., Ming, L.C., Petenate, A.J., Meireles, M. & Angela, A. Global yeilds, chemical composotions and antioxidant activities of Clove basil (Ocimum gratissimum L.). Journal of Food Processing Engineer. 2006, 29(5): 547559.
Singh, A.P. Relevance of polyherbal formulations. 2005). (website:www. ayurvedia.com on June 09, 2008).
King J: The Hydrolases Acid and Alkaline Ph osphatase. In Practical Clinical Enzymology . Edited by Van D. London: Nortstand Company Ltd; 1965:191 - 208.
Ugochukwu, N.H. & Babady, N.E. Antihyperglycemic effect of aqueous and ethanolic extracts of Gongronema latifolium leaves on glucose and glycogen metabolism in livers of normal and streptozotocin-induced diabetic rats. Life Science, 2003, 73(15): 1925-1938.
Ebong, P.E. Atangwho. I.J., Eyong, E.U. Ukere, C. Obi, A.U. Pancreatic beta cell regeneration. A probable parallel mechanism of hypoglycaemic action of Vernomia amygdalina and Azadirachta indica. Proceedings of International Neem Conferencce, Kuming, China. 2006, Nov. 11-12.
Sonia, B. and Scrinivasan, B.P. (Investigations into the anti-diabetic activity of Azadirachia indica. Indian Journal of Pharmacology, 1999, 31: 138 - 141.
Junge, W., Troge, B., Klein, G., Poppe, W., Gerber, M. Evaluation of new assay for pancreatic amylase: Performance characteristics and estimation of reference intervals. Clinical Biochemistry, 1989, 22(2): 109-14.
Kim, J.S., Ju, J.B., Choi, C.W. & Kim, S.C. Hypoglycemic and antihypenlipidemic effect of four Korean medicinal plants in alloxan induced diabetic rats. American Journal of Biochemistry and Biotechnology, 2006, 2 (4): 154-160.
Cunha, W.R., Arantes, G.M., Ferreira, D.S., Lucarini, R., Silva, M.L.A., Furtado, A.A., Crotti, A.E.M. & Aruju, A.R.B. Hypoglycemiceffect of Leandra locunosa in normal and alloxan induced diabetic rats. Fitoterapia, 2008, 79: 356-360
Pari, L. & Maheswari, J.U. Hypoglycoemia effect of Musa sapientum (Linn) in alloxaneinduced diabetic rats. Journal of Endoharmacology, 1999, 68: 321-325.
Eidi, A., Esmaeili, M. & Esmaeili, E. Antidiabetic effect of garlic (Allium sativum L.) in normal streptozotocininduced diabetic rats. Phytomedicine, 2006, 13: 624-629.
Sharma, B., Viswanath, G.,Salunke, R. & Roy, P. Effect of flavoniod – rich Extracts from seed of Eugenia jambolana (L.) on carbonhydrate and lipid metabolism in diabetic mice. Food Chemistry, 2008, 110: 697-705.
Djomeni, P.D.D., Tedong, L., Asongalem, E.A., Dimo, T., Siokena, S.D. & Kamtchouring, P. Hypoglycaemia and Antidiabetic effect of root extract of Ceiba pentandra in normal and diabetic rats. African Journal of Traditional Comp and Alternative Medicine, 2006, 3(1): 129-136.
Murray, R.R ., Granner, D.K., Mayes, P.A. & Roywell, V.W. Harper Biochemistry , 25 th Edition. Stamford Connecticuti Appleton and Lange, 2000, 610 - 617.
Rodrigues, B., Poucheret, P., Betell, M.L. & Mc Neill, J.N. Streptozotocin induced Diabetes: Induction, Mechanism(s), and Dose Dependency. In J. H. McNeill’s (Ed.), Experimental Models of Diabetes, 1999, pp. 3-17.
Leob, S. (Ed). Clinical laboratory Test: Values and Implicationns, Copyright edtion. Pennsylvania: Springhouse Corporation, Springhouse. 1991, pp. 498500.
Dunchen, L.W., Corselline, J.A. & Adams, J.H. Green fields neuropathology, 4 th edn. London, Edward Anold. 1984.
Gale, E.A.M. & Anderson, J.V., Diabetes Mellitus and other disorders of metabolism. In P. Kumas and M. Clark (eds.). Clini. Med. 4th Ed. W.B. Sunders, London, 1999 pp. 9591006.
Usoh, I. F. Akpan, H. D. Ekaidem, I.S., Uboh, F. E. Luke, U. O. Changes in blood glucose, body weights and serum lipids of streptozotocin-induced diabetic rats treated with combined leaf extracts of Gongronema latifolium and Ocimum gratissimum. European journal of Scientific Research 2015, 130(1): 68-81
Eleyinmi, A., Adebowale, Y., Oluwalana, I., Ajisafe, O. & Akintomide, T. Effect of Dietary Inclusion of Garcinia kola, Gongronema latifolium and Vernonia amygdalina on the Nutritional Quality of a Complementary Diet. Journal of Biological Sciences, 2006. 1: 43-49.
Eleyinmi, A., Sporns, P., Bressler, D., Nutritional Composition of Gongronema latifolium and Vernonia amygdalina. Journal of Nutrition and Food Science, 2008, 38: 99-109
Svensson, A. M., Ostenson, C. & Janssson, L. Age-induced Changes in Pancreatic Islet blood flow: Evidence for an impaired regulation in diabetes GK rats. American Journal Endocrinology and Metabolism, 2000, 279: 1139-1144.
Bolkent S, Yanardag R, Tabakoglu-Oguz A, Ozsoy-Sacan O: Effects of Chard extract (Beta vilgaris L. var. Cicla) extract on pancreatic beta-cells in streptozotocin – diabetic rats: a morphological and biochemical study. J Ethanopharmacol 2000, 73:251 – 259.
Jelodar, G., Mohsen, M. & Shahram, S. Effect of walnut leaf, coriander and pomegranate on blood glucose and histopathology of pancreas of alloxan induced diabetic rats. African Journal of Traditional, complementary and A item alive Medicines, 2007, 4(3): 299-3 05.
Noor, A., Gunasekeran, S., Manicckam, A. S. & Vijayaiakshmi, M. A. Antidiabetic activity of Aleo vera and histology of organs in streptozotocin-induced diabetic rats. Current Science, 2008, 94(8): 10701076.
Yeh, G. Y., Eisenberg, D.M., Kaptcul, T.J and Philips R.S. Systemic review of herbs and dietary supplements for glycemic control in diabetes. Diabetes Care. 2003, 26 (4), 1277-1294.
Usoh, I. F., Akpan, H. D. and Akpanyung, E. O., Combined Phytochemicals from Gongronema latifolium and Ocimum gratissimum Leaves Extracts Potentiate in vitro Free Radical Scavenging. IOSR International Journal of Pharmacy and Biological Sciences 2015, 10(5):68-74.
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