Anti-inflammatory activity of Mi LABS Joint Support supplement (MLJS-01) in RAW 264.7 cell lines

Abstract

The aim of this study was to investigate the effect of Mi LABS Joint Support supplement (MLJS-01) on the production of TNF-α, an important inflammatory mediator, in RAW 264.7 cell lines. Isolated RAW 264.7 cells were activated with 1 µg/ml lipopolysaccharide in the presence of MLJS-01 (25 and 50 µg/ml) and Dexamethasone (200 µM). Tumor necrosis factor-α secretion was quantified after 4h by L-929 bioassay. MLJS-01 showed dose dependent inhibition (31.19±3.40 and 24.75±4.82% at 50 and 25μg/ml, respectively) of LPS-induced TNF-α production in RAW 264.7 cell lines. The reduced production of TNF-α in RAW 264.7 cell lines may be involved in anti-inflammatory activity conveyed by MLJS-01.