Some properties of protease extract derived from Pseudomonas fluorescens and its antifungal activity against Fusarium spp.

Authors

  • Yati Sudaryati Soeka Research Center For Biology-Indonesian Institute of Sciences Cibinong Science Center, Jl. Raya Jakarta- Bogor, Km 46 Cibinong 16911, Indonesia
  • Joko Sulistyo Faculty of Food Science and Nutrition, University Malaysia Sabah, Jalan UMS 88400, Kota Kinabalu, Sabah, Malaysia

DOI:

https://doi.org/10.56511/JIPBS.2018.v5.i04.pp104-110

Abstract

Soil-borne plant pathogenic fungi are of major concern problem in agriculture which affects yield and quality of agricultural products. As Pseudomonas fluorescens possess a variety of promising properties which make it a better biocontrol agent. In this current study, antagonistic effects of bacterial biocontrol agent, Pseudomonas fluorescens obtained from Microbiology Collection of Indonesian Institute of Sciences, was evaluated against plant pathogenic fungi Fusarium solani and Fusarium oxysporum causing wilt disease of plants. The ability of Pseudomonas fluorescens in antagonizing or inhibiting the growth of phytopathogenic fungi was tested by measuring the inhibition zone for the growth of the tested fungi using dual culture method. The protease extract derived from P. fluorescens showed to inhibit growth of Fusarium solani InaCC F76 and Fusarium oxysporum InaCC F78 as pathogens cause wilt disease on plants

Keywords:

Pseudomonas fluorescens, Fusarium oxysporum, Fusarium solani, protease extract, antifungal

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References

Erickson B , Nelson JE and Winters P . Perspective on opportunities in industrial biotechnology in renewable chemicals. Biotechnol ogy J ournal 2012 ; 7 (2) : 176 – 185 .

Ajmal M, Ali HI, Saeed R, Akhtar A, Tahir M, Mehboob MZ and Ayub A. Biofertilizer as an Alternative for Chemical Fertilizers. Research & Reviews: Journal of Agriculture and Allied Sciences 2018; 7(1): 1-7.

Nihorembere V, Ongena M, Smargiass M, Beneficial effect of the rhizosphere microbial community for plant growth and health. Biotechnol.Agron. Soc. Environ. 2011; 15 (2): 327-337.

Gupta CP, Dubey RC, Kang SC, Maheh Wari DK. Antibiosis-mediated necrotrophic effect of Pseudomonas GRC2 against two fungal plant pathogens. Current Science 2001; 81: 91-94.

Ursula SK, Arnaud S, Monika M, Caroline B, Brion D, Cecile G.B, Cornelia R, Regina N, Genevie VDF, Dieter H and Christoph KL. Autoinduction of 2, 4 Diacetylphloroglucinol biosynthesis in biocontrol agent Pseudomonas fluorescens CHAO and repression by the bacterial metabolites salicylate and pyoluteorin. Journal of Bacteriology 2000; 182(5): 1215-1225.

Munkvold GP. Epidemiology of Fusarium diseases and their mycotoxins in maize ears. European Journal of Plant Pathology. 2003; 109: 705 – 713.

Franks A, Ryan RP, Abbas A, Mark GL, and O’Gara F Molecular tools for studying plant growth promoting rhizobacteria. In: Cooper JE, Rao JR (eds) Molecular approaches to soil, rhizosphere and plant microorganisms analysis. Biddes Ltd, Kings Lynn, UK 2006; 116 – 131.

Shalini RS. Antifungal Activity of Pseudomonas fluorescens against different plant pathogenic fungi. The Internet Journal of Microbiology 2008; 7(2): 1-7.

Enya J, Togawa M, Takeuchi T, Yoshida S, Tsushima S, Arie T and Sakai T. Biological and Phylogenetic Characterization of Fusarium oxysporum Complex, Which Causes Yellows on Brassica spp., and Proposal of F. oxysporum f. sp. rapae, a Novel Forma Specialis Pathogenic on B. rapa in Japan. Phytopathology 2008; 98(4): 475-483.

Naiola E, and Widhyastuti N. Semi purification and characterization of protease from Bacillus sp. Journal of Periodic Biological Research 2007; 13(1): 51-56 ( in Indonesian).

Cappuccino JG and Sherman N. Microbiology: A Laboratory Manual. AddisonWesley Publishing Company. California USA. 1983.

Yang SS and Huang CI. Proteases production by amylolytic fungi in solid state fermentation. Journal of Chinese Agricultural Chemical Society 1994; 32(6): 589 - 601.

Susanti EVH. Isolation and Characterization of Proteases from Bacillus subtilis 1012M15. Jurnal Biodiversitas 2003 ; 4(1) : 12 - 17. (in Indonesian).

Correa APF, Daroit DJ, Velho RV and Brandelli A. Hydrolytic potential of a psychrotrophic Pseudomonas isolated from refrigerated raw milk. Brazilian Journal of Microbiology 2011; 42: 1479-1484.

Pakpahan R. Isolation of bacteria and testing of thermophilic protease activity from hot water sources Sipoholon North Tapanuli, Northern Sumatra . Postgraduate School, University of North Sumatra, Medan. [ Thesis ]. 2009 . (in Indonesian).

Soeka YS. Optima zation of lipase from Pseudomonas fluorescen s . First Edition Pro ceeding of National Seminar on Biodivers ity , Airlangga University, Surabaya . 201 6. (in Indonesian).

Patil P, Sabale S and Devale A. Isolation and Characterization of Protease Producing Bacteria from Rhizosphere Soil and Optimization of Protease Production Parameters. International Journal of Current Microbiology and Applied Sciences 2015 ; 2 : 58 - 64.

Wiedmann M, Weilmeier D, Dineen SS, Ralyea R and Boor KJ. Molecular and phenotypic characterization of Pseudomonas spp. isolated from milk. Applied and Environmental Microbiology 2000 ; 66(5) : 2085 - 2095.

Dogan B and Boor KJ. Genetic diversity and spoilage potentials among Pseudomonas spp. isolated from fluid milk products and dairy processing plants. Applied and Environmental Microbiology 2003 ; 69(1) : 130 - 138.

Dufour D, Nicodème M, Perrin C , Driou A , Brusseaux E, Humbert G, Gaillard J-L and Dary A . Molecular typing of industrial strains of Pseudomonas spp. isolated from milk and genetical and biochemical characterization of an extracellular pr otease produced by one of them. International Journal of Food Microbiology 2008 ; 125(2) : 188 - 196.

Rauf A, Irfan M., Naleem M., Ahmed I. and Iqbal HMN. Optimization of growth conditions for acidic protease productions from Rhizopus oligosporus through solid state fermentation of sunflower meal. International Journal of Biological, Biomolecular, Agricultural, Food and Biotechnological Engineering 2010; 4(12): 898-901.

Bisswanger H. Enzyme assays. Perspectives in Science 2014; 1(1 – 6 ): 4155.

Rodarte MP, Dias DR, Vilela DM and Schwan RF . Proteolytic activities of bacteria, yeasts and filamentous fungi isolated from coffee fruit ( Coffea arabica L . ). Acta Scientiarum Agronomy Maringá 2011 ; 33(3) : 457 - 464.

Zhang WW, Hu YH, Wang HL and Sun L. Identification and characterization of a virulence-associated protease from a pathogenic Pseudomonas fluorescens strain. Veterinary Microbiology 2009; 139 (1-2): 183 – 188.

Robinson PK. Enzymes: principles and biotechnological applications. Essays Biochemistry 2015; 59: 1 – 41.

Wang L and Jayarao BM. Phenotypic and genotypic characterization of Pseudomonas fluorescens isolated from bulk tank milk. Journal of Dairy Science 2001; 84(6): 1221-1229.

George-Okafor UO and Mike-Anosike EE. Screening and optimal protease production by Bacillus sp. Sw-2 using low cost substrate medium. Research Journal of Microbiology 2012; 7(7): 327-336.

Bacha E, Sant’Anna V, Daroit DJ, Correa APF, Segalin J, Brandelli A. Production, one-step purification, and characterization of a keratinolytic protease from Serratia marcescens P3. Process Biochemistry 2012; 47: 2455 – 2462.

Gupta R, Beg QK and Lorenz P. Bacterial alkaline proteases: molecular approaches and industrial applications. Applied Microbiology and Biotechnology 2002 ; 59(1) : 15 - 32.

Sabotic J and Kos J . Microbial and fungal protease inhibitors - current and potential applications. Applied Microbiology and Biotechnology 2012 ; 93(4) : 1351 - 1375.

Oh YS, Shih IL, Tzeng, YM, Wang, SL. Protease produced by Pseudomonas aeruginosa K-187 and its application in the deproteinization of shrimp and crab shell wastes. Enzyme and Microbial Technology. 2000; 27(1): 3-10.

Zambare V , Nilegaonkar S and Kanekar P . A novel extracellular protease from Pseudomonas aeruginosa MCM B - 3 27: enzyme production and its partial characterization . New Biotechnology 2 011 ; 28(2): 173 – 181.

Koka R and Weimer BC. Isolation and characterization of a protease from Pseudomonas fluorescens RO98. Journal of Applied Microbiology 2000 ; 89 : 280 - 288.

Chen L, Daniel RM, Coolbear, T. Detection and impact of protease and lipase activities in milk and milk powders. International Dairy Journal 2003; 13(4): 255-275.

Nielsen SS. Plasmin system and microbial proteases in milk: characteristics, roles, and relationship. Journal of Agriculture and Food Chemistry 2002; 50(22): 6628-6634.

Stepaniak L. Dairy enzymology. International Journal of Dairy Technology 2004; 57(1-2): 153-171.

Sattar H, Aman A and Ul Qader SA. Effect of Metal Ions, Solvents and Surfactants on the Activity of Protease from Aspergillus niger KIBGEIB36 Journal of Basic & Applied Sciences 2017; 13:491-495.

Priebatsch KM, Kvansakul M, Poon IKH and Hulett MD. Functional regulation of the plasma protein histidine-rich glycoprotein by Zn 2+ in settings of tissue injury. Biomolecules 2017; 7(1): 1-25.

Martins ML, Pinto UM, Riedel K and Vanetti MCD. Milk - deteriorating exoenzymes from Pseudomonas fluorescens 041 isolated from refrigerated raw milk. Brazilian Journal of Microbiology 2015 ; 46(1) : 207 - 217.

Blaby-Haas CE and Merchant SS. Lysosome-related Organelles as Mediators of Metal Homeostasis. Journal of Biological Chemistry 2014; 289(41): 28129 – 28136.

Rajmohan S , Dodd CE, and Waites WM. Enzymes from isolates of Pseudomonas fluorescens involved in food spoilage. Journal of Applied Microbiology 2002; 93(2), 205-213.

Wi dhyastuti N , Hotimah B and Achmadi SS. Properties of extracellular protease of bacterial P.1 strain isolated from palm wine . Indon esia n J urnal for Biol og y 2001 ; 3(1): 80 - 89 . (in Indonesian).

Baehaki A, Nurhayati , T and Suhartono MT. Characteristics of protease from pathogenic bacteria Staphylococcus epidermidis . Bulletin of Fisheries Product Technology 2005 ; 8 ( 2 ); 25 - 34. (in Indonesian).

Li Q, Yi L, Marek P, and Iverson BL. Commercial proteases: Present and future. FEBS Letters . 2013; 587(8): 1155 – 1163.

Heydari A and Pessarakli M. A review on biological control of fungal plant pathogens using microbial antagonists. Journal of Biological Sciences. 2010; 10(4): 273-290.

Zehnder GW, Murphy JF, Sikora EJ, Kloepper JW. Application of rhizobacteria for induced resistance. European Journal of Plant Pathology. 2001; 107(1): 39-50.

Showkat S, Murtaza I, Laila O and Ali A. Biological control of Fusarium oxysporum and Aspergillus sp. by Pseudomonas fluorescens isolated from wheat rhizosphere soil of Kashmir. IOSR Journal of Pharmacy and Biological Sciences 2012; 1(4): 24-32.

Chernin L and Chet I. Microbial enzymes in biocontrol of plant pathogens and pests. R.G. Burns, R.P. Dick, Eds., Enzymes in the environment: activity, ecology, and applications. Marcel Dekker, inc., New York. 2002: 171 – 225.

Huang CJ, Wang TK, Chung SC, and Chen CY. Identification of an antifungal chitinase from a potential biocontrol agent, Bacillus cereus 28 –

Journal of Biochemistry and Molecular Biology 2004; 38(1): 82 – 88.

Hoster F, Schmitz JE, Daniel R. Enrichment of chitinolytic microorganisms isolation and characterization of a chitinase exhibiting antifungal activity against phytopathogenic fungi from a novel Streptomyces strain. Applied Microbiology and Biotechnology 2005; 66: 434 – 442.

Aktuganov GE, Galimzyanova NF, Melent’ Ev AI, and Kuz’ Mina LY. Extracellular hydrolases of strain Bacillus sp.739 and their involvement in the lysis of micromycetecell walls. Mikrobiologiia 2007; 76(4): 471 – 479.

Bogas AC, Watanabe MAE, Barbosa A, Vilas-Boas LA, Bonatto AC, Dekker R, Souza EM, and Fungaro MHP. Structural characterization of the bglH gene encoding a beta glucosidase-like enzyme in an endophytic Bacillus pumilus strain. Genetics and Molecular Biology 2007; 30(1): 100 – 104.

Jankiewicz U, Goł ą b D and Fr ą k M. Effect of salicylic acid, synthesized by the bacteria Pseudomonas fluorescens and P. chlororaphis on phytopathogenic fungi Fusarium. Polish Journal of Agronomy 2013; 15: 65 – 68.

Koche MD, Gade RM and Deshmukh AG. Antifugal activity of secondary metabolites produced by Pseudomonas fluorescens. The Bioscan. Supplement on Medicinal Plants 2013; 8 (2): 723-726.

Toua D, Benchabane M, Bnsaid F and Bakour R. Evaluation of Pseudomonas fluorescens for the biocontrol of fusarium wilt in tomato and flax. Glob J Microbiol Res. 2013; 1(1): 075-084.

Published

05-10-2018
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How to Cite

Yati Sudaryati Soeka, and Joko Sulistyo. “Some Properties of Protease Extract Derived from Pseudomonas Fluorescens and Its Antifungal Activity Against Fusarium Spp”. Journal of Innovations in Pharmaceutical and Biological Sciences, vol. 5, no. 4, Oct. 2018, pp. 104-10, doi:10.56511/JIPBS.2018.v5.i04.pp104-110.

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Research Article