In vitro phytochemical analysis and cytotoxic assay of leaves of Solanumlycopersicum Linn by brine shrimp bioassay

Authors

  • Homaira Afreen Manarat International University, Dhaka, Bangladesh
  • Toufiq-Ul Amin Southeast University, Dhaka, Bangladesh
  • Md. Siddiqul Islam Southeast University, Dhaka, Bangladesh
  • Salma Parvin Manarat International University, Dhaka, Bangladesh
  • Rayhanus Salam Southeast University, Dhaka, Bangladesh

DOI:

https://doi.org/10.56511/JIPBS.2016.v3.i03.pp81-85

Abstract

The aim of this study is to phytochemically analyze and thus identify the constituents present in the leave extract of tomato (Solanumlycopersicum Linn). Besides, biological activity of the crude extract was investigated with special emphasis to the brine shrimp lethality bioassay to figure out the potential cytotoxic activity. Identification of Reducing sugar, Tannin, Flavonoids, Gum, Steroid, Saponin and Alkaloid was confirmed by testing of different chemical groups present in the methanolic extract represent the preliminary phytochemical studies. In the brine shrimp lethality bioassay, in minimum concentrations LC50 value was found to be 0.69mg/ml for crude dichloromethane partition at extract, which revealed potential cytotoxic activity.  

Keywords:

olanumlycopersicum, Brine Shrimp, Phytochemical Analysis, Cytotoxic, Bioassay

Downloads

Download data is not yet available.

References

Yousaf, Z., Wang, Y., Baydoun, E.: Phytochemistry and pharmacological studies on Solanum torvum Swartz. Journal of Applied Pharmaceutical Science 2013; 3(4):152-160.

Biosci, J.: Krishna, J. MuraliPhytochemical Analysis and Antimicrobial Studies of Various Extracts of Tomato (Solanum lycopersicum L.). Journal: Sch. Acad. J. Biosci 2013; 4.

Ahmed, B., Zayed, R., Ahmed, S., Hassanean, H.: Phytochemical and pharmacological studies of Solanum elaeagnifolium growing in Egypt. J Nat Prod 2013; 6:156-67.

Edmonds, J., Chweya, J.: Black nightshades: Solanum nigrum L. and related species. 15. Bioversity International 1997.

Hu, K., Kobayashi, H., Dong, A., Jing, Y., Iwasaki, S., Yao, X.: Antineoplastic agents III: steroidal glycosides from Solanum nigrum. Planta medica 1999; 65(01):035038.

Gautam, G., Vidyasagar, G., Dwivedi, S.: Study on Medicinal Plants from Indian Origin. Lambert Academic Publishing 2012.

Dewanto, V., Wu, X., Adom, K., Liu, R.: Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. Journal of agricultural and food chemistry 2002; 50(10):3010-3014.

Holiman, P., Hertog, M., Katan, M.: Analysis and health effects of flavonoids. Food Chemistry 1996; 57(1):43-46.

Rao, A., Agarwal, S.: Role of lycopene as antioxidant carotenoid in the prevention of chronic diseases: a review. Nutrition research 1999; 19(2):305-323.

Agarwal, A., Shen, H., Agarwal, S., Rao, A.: Lycopene content of tomato products: its stability, bioavailability and in vivo antioxidant properties. Journal of medicinal food 2001; 4(1):9-15.

Abushita, A., Daood, H., Biacs, P.: Change in carotenoids and antioxidant vitamins in tomato as a function of varietal and technological factors. Journal of Agricultural and Food Chemistry 2000; 48(6):2075-2081.

Vanwagenen, B. C., Larsen, R., Cardellina, J. H., Randazzo, D., Swithenbank: Ulosantoin, a potent insecticide from the sponge Ulosa ruetzleri. J. Org. Chem. 1993; 58:335-337.

Vohora, S., Kumar, I.: STUDIES ON TAXUS BACCATA – I. Preliminary Phytochemical and Behavioral Investigation. Planta medica 1971; 20(4):100-107.

Yadav, R., Agarwala, M.: Phytochemical analysis of some medicinal plants. Journal of Phytology 2011; 3(12):10-14.

Medina, J., D, R., Coussio, J.: Phytochemical screening of argentine plants with potential pharmacological activity (part vii) 1. Planta medica 1977; 31(2):136-140.

Abdel-Halim, Bashir, O., Morikawa, T., Ando, S., Matsuda, H., Yoshikawa, M.: New crinine-type alkaloids with inhibitory effect on induction of inducible nitric oxide synthase from Crinum yemense. Journal of natural products 2004; 67(7):1119-1124.

Meyer, B. N., Ferringni, N. R., Puam, J. E., Lacobsen, L. B., Nichols, D. E., McLaughlin, J. L.: Brine shrimp: a convenient general bioassay for active constituents. Planta Med. 1982; 45:31-32.

Published

05-07-2016
Statistics
218 Views | 165 Downloads

How to Cite

Homaira Afreen, Toufiq-Ul Amin, Md. Siddiqul Islam, Salma Parvin, and Rayhanus Salam. “In Vitro Phytochemical Analysis and Cytotoxic Assay of Leaves of Solanumlycopersicum Linn by Brine Shrimp Bioassay”. Journal of Innovations in Pharmaceutical and Biological Sciences, vol. 3, no. 3, July 2016, pp. 81-85, doi:10.56511/JIPBS.2016.v3.i03.pp81-85.

Issue

Volume 3, Issue 3, 2016 (Jul-Sep)

Section

Research Article

Copyright (c) 2016 Homaira Afreen, Toufiq-Ul Amin, Md. Siddiqul Islam, Salma Parvin, Rayhanus Salam

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.