In vitro phytochemical analysis and cytotoxic assay of leaves of Solanumlycopersicum Linn by brine shrimp bioassay

Abstract

The aim of this study is to phytochemically analyze and thus identify the constituents present in the leave extract of tomato (Solanumlycopersicum Linn). Besides, biological activity of the crude extract was investigated with special emphasis to the brine shrimp lethality bioassay to figure out the potential cytotoxic activity. Identification of Reducing sugar, Tannin, Flavonoids, Gum, Steroid, Saponin and Alkaloid was confirmed by testing of different chemical groups present in the methanolic extract represent the preliminary phytochemical studies. In the brine shrimp lethality bioassay, in minimum concentrations LC₅₀ value was found to be 0.69mg/ml for crude dichloromethane partition at extract, which revealed potential cytotoxic activity.