HPTLC method for simultaneous estimation of Metformin HCl and Sitagliptin in pharmaceutical dosage form

Abstract

An accurate, precise and derivatized HPTLC method has been developed for the simultaneous estimation of Metformin and Sitagliptin in tablet formulation. In this method standard and sample solutions of Metformin and Sitagliptin  were applied on pre-coated silica gel 60F₂₅₄ TLC plate, and developed using Ammonium sulphate (0.5%)-2-Propanol-Methanol (8:1.6 :1.6v/v ), as mobile phase[1] and derivatized using CAMAG-REPROSTAR-3. A camag HPTLC system comprising of Camag Linomat-5-applicator, camag twin trough chamber, camag reprostar-3, camag TLC -3- scanner was used for the analysis. The drugs on the plate were scanned at 254 nm. The dynamic linearity range was 700-1500 ng/spot for Sitagliptin and 7-15μg/spot for Metformin. The method was validated for precision, accuracy and reproducibility