Study the effect of hairy root transformation on rapid growth (growth morphology) of Nepeta cataria in vitro cultures
DOI:
https://doi.org/10.56511/JIPBS.2015.v2.i04.pp439-450Abstract
In the current study, an efficient protocol for in vitro plant regeneration and genetically transformed root induction by agrobacterium rhizogenes strain A4 was developed. Powerful response for hairy roots induction was achieved using stem explants cultured on co-cultivation half strength MS-medium containing 500 mg/l cefotaxime. Contrary leaf explants does not have any response for hairy roots induction. Maximum shoot number (23.601 ± 2.073) and root number (13.604 ± 1.673) were investigated in the transformed plants with lengths 21.611 ± 2.073 cm and 10.923 ± 0.741cm, respectively. The highest value of hairy growth (1.506 ± 0.104 g) was attained after 15 days of Culture. Hairy root lines were successfully confirmed by PCR using the rolB gene. Collectively, our results demonstrate that we have successfully used the methodology of transformation to induce hairy root culture of N. cataria and facilitate a valuable alternative approach for regeneration a genetically transformed roots
Keywords:
Nepeta cataria, Seed germination, Agrobacterium rhizogenes A4, Hairy root transformation, PCR analysisDownloads
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