A multi-component spectrophotometric method for simultaneous determination of conjugated bilirbuin, unconjugated bilirubin, oxyhemoglobin and methemalbumin in adult human serum

Authors

  • Atef M. M. Attia Biochemistry Department, National Research Centre, 33 Bohouth Street, Dokki, Giza, Egypt (Affiliation ID: 60014618)
  • Abd El-Baset M. Sayed Biochemistry Department, National Research Centre, 33 Bohouth Street, Dokki, Giza, Egypt (Affiliation ID: 60014618)
  • Wael M. Aboulthana Biochemistry Department, National Research Centre, 33 Bohouth Street, Dokki, Giza, Egypt (Affiliation ID: 60014618)
  • Fatma A. A. Ibrahim Biochemistry Department, National Research Centre, 33 Bohouth Street, Dokki, Giza, Egypt (Affiliation ID: 60014618)
  • Noha A. Abd El-Latif Biochemistry Department, National Research Centre, 33 Bohouth Street, Dokki, Giza, Egypt (Affiliation ID: 60014618)
  • Samir W. Aziz Biochemistry Department, National Research Centre, 33 Bohouth Street, Dokki, Giza, Egypt (Affiliation ID: 60014618)
  • Azhar M. Elwan Biochemistry Department, National Research Centre, 33 Bohouth Street, Dokki, Giza, Egypt (Affiliation ID: 60014618)
  • Mohamed D. E. Abdelmaksoud Biochemistry Department, National Research Centre, 33 Bohouth Street, Dokki, Giza, Egypt (Affiliation ID: 60014618)
  • Sherif A. A. Moussa Biochemistry Department, National Research Centre, 33 Bohouth Street, Dokki, Giza, Egypt (Affiliation ID: 60014618)

DOI:

https://doi.org/10.56511/JIPBS.2020.v7.i03.pp01-06

Abstract

A new multi-component spectrophotometric method was developed to determine the accurate concentrations of the conjugated bilirubin (CB), unconjugated bilirubin (UB), oxyhemoglobin (HbO2) and methemalbumin (Mha) in adult human serum of a healthy subject. The method of preparation of serum solutions has been developed, like the use of distilled water as a solvent and centrifugation of serum solutions to clear the sample turbidity. The results of bilirubins were compared to the diazo assay, as a reference method. The formulas used for the calculation of the major components (UB, CB, HbO2 and Mha) in human serum have been derived based on the theory of multi-component spectrophotometric analysis and the mathematical Gaussian elimination method for matrix calculation. The method of multi-component spectrophotometry (at λ=450, 490, 576, 600 and 700 nm), suggested in this study for determination of total bilirubin (TB), UB and CB showed small % error of values 0.201, 2.772 and 7.81%, respectively, indicating the high accuracy of the method. The method showed small coefficients of variation (CV=3.84-10.5%), indicating the high precision of the method. The method is inexpensive, precise, accurate, and reproducible and has the advantages of small sample volume, simplicity speed and can be computerized.

Keywords:

Total bilirubin, unconjugated bilirubin, conjugated bilirubin, oxyhemoglobin (HbO2), methemalbumin (Mha), serum, adults

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Published

01-07-2020
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How to Cite

Atef M. M. Attia, A. E.-B. M. Sayed, Wael M. Aboulthana, Fatma A. A. Ibrahim, Noha A. Abd El-Latif, Samir W. Aziz, Azhar M. Elwan, M. D. E. Abdelmaksoud, and Sherif A. A. Moussa. “A Multi-Component Spectrophotometric Method for Simultaneous Determination of Conjugated Bilirbuin, Unconjugated Bilirubin, Oxyhemoglobin and Methemalbumin in Adult Human Serum”. Journal of Innovations in Pharmaceutical and Biological Sciences, vol. 7, no. 3, July 2020, pp. 01-06, doi:10.56511/JIPBS.2020.v7.i03.pp01-06.

Issue

Volume 7, Issue 3, 2020 (Jul-Sep)

Section

Research Article

Copyright (c) 2020 Atef M. M. Attia, Abd El-Baset M. Sayed, Wael M. Aboulthana, Fatma A. A. Ibrahim, Noha A. Abd El-Latif, Samir W. Aziz, Azhar M. Elwan, Mohamed D. E. Abdelmaksoud, Sherif A. A. Moussa

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