Development of a spectrophotometric method for the assay of Diclofenac potassium

Authors

  • Safila Naveed Faculty of Pharmacy Jinnah University for women, Karachi , Pakistan
  • Hina Rehman Faculty of Pharmacy Jinnah University for women, Karachi , Pakistan
  • Fatima Qamar Faculty of Pharmacy Jinnah University for women, Karachi , Pakistan
  • Syeda Zainab Faculty of pharmacy, University of Karachi, Pakistan
  • Kamran Alam Faculty of Pharmacy Jinnah University for women, Karachi , Pakistan
  • Syed Junaid Rehan Faculty of Pharmacy Jinnah University for women, Karachi , Pakistan

DOI:

https://doi.org/10.56511/JIPBS.2015.v2.i01.pp07-11

Abstract

Diclofenac potassium is a non- steroidal anti-inflammatory drug and a prostaglandin (PG) synthetase inhibitor. The objective of this study was to develop a spectrophotometric method for the assay of diclofenac potassium. A series of diclofenac potassium solutions were prepared ranging from 200 ppm to 12.5 ppm. After preparation of a standard stock solution of 200 ppm in 100 ml of water, different dilutions were made (100ppm, 50ppm, 25ppm and 12.5ppm). At the wavelength 242nm, the absorbance of the standard preparation and dilutions was measured using a UV spectrophotometer. In this study a precise and accurate method was developed. The validation of the developed method for the assay of diclofenac potassium was done by various parameters that included linearity, accuracy test and precision. The method showed good reproducibility and good recovery with % RSD less than 2. The method was found to be simple, economical, rapid, specific, precise and accurate.

Keywords:

Diclofenac Potassium, accuracy, linearity, regression equation, precision

Downloads

Download data is not yet available.

References

W arner TD, Giuliano F, Vojnovic I, Bukasa A, Mitchell JA, Vane JR. Nonsteroid drug selectivities for cyclo - oxygenase - 1 rather than cyclo - oxygenase - 2 are associated with human gastrointestinal toxicity: A full in vitro analysis. Proc Natl Acad Sci U S A. 1999; 96:7563 – 8.

Frolich JC. A classification of NSAIDs according to the relative inhibition of cyclooxygenase isoenzymes. Trends Pharmacol Sci. 1997; 18 :30 – 4.

Bianchi M. Are all NSAIDs other than ‘coxibs’ really equal? Trends Pharmacol Sci. 2004; 25:6 – 7.

Chantler I, Mitchell D, Fuller A. The effects of three cyclo - oxygenase inhibitors with different cyclo - oxygenase - 2 specificity on intensity of primary dysmenorrhoeic pain. Clin J Pain. 2008; 24:39 – 44.

Chantler I, Mitchell D, Fuller A. Diclofenac potassium attenuates dysmenorrho eic pain and restores exercise performance in women with primary dysmenorrhoea. J Pain. 2008 In press.

Khatal LD, Kamble AY, Mahadik MV, Dhaneshwar SR.Validated HPTLC method - day Intra - day %Recovery %RSD %Recovery 101.07 0.51 98.00 102.24 1.2 100.00 100.56 1.92 100.00 101.04 1.72 100.40 100.65 1.11 100.40 Table No. 3: Precision of Diclofenac Potassium for simultaneous quantitation of paracetamol, diclofenac potassium, and famotidin e in tab let formulation. 2010.J AOAC Int.93(3):765 - 70.doi: 10.5555/jaoi.93.3.765

Umarkar AR , Mehta SA ,Chaple DR and Thote LT, Simultaneous Estimation of Famotidine and Diclofenac Potassium by UV Spectrophotometer Using Multicomponent Method.Journal of Ph armacy Research. 2011. Vol 4, No 4.

Jadhav SD, Butle SR, Patil SD, Jagtap PK. Validated stability indicating RP - HPLC method for simultaneous determination and in vitro dissolution studies of thiocolchicoside and diclofenac potassium from tablet dosage form . Arabian Journal of Chemistry.2011.doi: 10.1016/j.arabjc.2011.01.0118.

Elkady EF.Simultaneous determination of diclofenac potassium and methocarbamol in ternary mixture with guaifenesin by reversed phase liquid chromatography. Talanta. 2010. 82(4), 1604 - 16 07 doi:10.1016/j.talanta.2010.07.024

Gowramma B, Rajan S, Muralidharan S, Meyyanathan SN and Suresh B. A validated RP - HPLC method for simultaneous estimation of Paracetamol and Diclofenac Potassium in pharmaceutical formulation. International Journal of ChemTech Research.2010. Vol.2, No.1, pp 676 - 680.

Umarkar AR, Rewatkar NS, Charde MS, Charde RM.Simultaneous Estimation of Thiocolchicoside and Diclofenac Potassium by UV Spectrophotometer Using Multicomponent Method. International Journal of ChemTech Resea rch. 2011. Vol. 3, No.2, pp 944 - 947.

Umarkar AR,Yogesh BM, Mayur BR, Pravin KS, Absorption Correction Method for Estimation of Thiocolchicoside and Diclofenac Potassium in Combined Capsule Dosage Form. International Journal of Pharmaceutical Sciences 2011: 3(1):1046 -

Published

05-01-2015
Statistics
256 Views | 352 Downloads

How to Cite

Naveed, S., H. Rehman, F. Qamar, S. Zainab, K. Alam, and S. J. Rehan. “Development of a Spectrophotometric Method for the Assay of Diclofenac Potassium”. Journal of Innovations in Pharmaceutical and Biological Sciences, vol. 2, no. 1, Jan. 2015, pp. 7-11, doi:10.56511/JIPBS.2015.v2.i01.pp07-11.

Issue

Volume 2, Issue 1, 2015 (Jan-Mar)

Section

Research Article

Copyright (c) 2015 Safila Naveed, Hina Rehman, Fatima Qamar, Syeda Zainab, Kamran Alam, Syed Junaid Rehan

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.