An alkaloid derivative from Coscinium fenestratum exhibit dual COX/LOX inhibition
DOI:
https://doi.org/10.56511/JIPBS.2018.v5.i01.pp117-121Abstract
Agents that inhibit both Cyclooxygenase (COX) and Lipoxygenase (LOX) are highly recommended in the development of anti-inflammatory drugs. An alkaloid derivative from Coscinium fenestratum was isolated and characterized by UV Visible, IR, 1H NMR, 13C NMR and LC-MS spectroscopy. Dual COX/LOX inhibitory study was performed by high throughput screening assay. In the control (maximum enzyme activity) experiment COX activity of 73 µmol/min was noted, which is reduced to 50 µmol/min by the compound isolated. The standard inhibitors show 38 and 29 µmol/min respectively for Aspirin and Nimesulide. Compound isolated from Coscinium fenestratum also possess significant LOX inhibition when compared to standard inhibitor vanillin.
Keywords:
Coscinium fenestratum, Cyclooxygenase, Lipoxygenase, Aspirin, NimesulideDownloads
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