In vitro hemostatic activity of ethanol extracts of Beetroot (Beta vulgaris L.) in blood male albino rat
DOI:
https://doi.org/10.56511/JIPBS.2018.v5.i01.pp34-36Abstract
Objective: The purpose of this research was to determine the in vitro hemostatic activity of ethanol extract of beetroot (Beta vulgaris L). Methods: In vitro hemostatic activity was performed on whole blood of rat by Lee-White method to determine the clotting time and the Eustrek method to observe the microscopic picture of clotted blood. Results: The results of in vitro study showed that ethanol extract of Beetroot at the concentration of 1% and 2% decreased the clotting time at the minute of 24.4+1.14 and 15.9 + 0.65, respectively, as compared to EDTA treatment which did not clot for 120 min (p<0.05) and microscopically showed that blood cells appear to be attached to each other at the concentration of 1% and 2% of ethanol extract of Beetroot. The hemostatic activity of ethanol extract of beetroot showed a dose-dependent manner in the in vitro study. Conclusion: Ethanol extract of beetroot (Beta vulgaris L) has hemostatic activity on in vitro method.
Keywords:
Beetroot (Beta vulgaris L.), Hemostatic, Lee-white, EustrekDownloads
References
Janz G , and Glenn CH . Disorders of Hemostasis . Timothy Medicine and Surgery 2012; 1606
Boon GD . An Overview of Hemostasis. Toxicologic Pathology 1993; 21:170 - 180
Mehta A, and Hoffbrand AV . At A Glance Hematology 2nd ed ition . Jakarta: Erlangga ; 2006 : 73.
Mitra R: Medicinal Plants of indonesia 2007. APBN; 11: 11
Odoh UE and Okor o EC . Quantitative Phytochemic a l, Proximate/Nutritive Composition Analysis o f Beta vulgaris Linnaeus (Chenopodiaceae) . International Journal o f Current Research 2013 ; 5: 3723 - 3728 .
Palta S, Saroa R, and Palta A . Overview of the coagulation system. Indian J Anaesth 2014; 58(5): 515 – 523.
Indhumathi T, and Kannikaparameswari K. Hematopoietic Study o f The Methanolic Root Extract of Beta vulgaris on Albino Rats - An In Vivo Study. Int J Pharm Bio Sci 2012 ; 3(4) : 1005 – 1015 .
Depkes RI . Materia Medika. 6th E d ition . Jakarta: Ditjen POM; 1995 ; 297 - 307.
Farnsworth NR . Biologycal and phytochemical screening of plants. J Pharm Sci 1996; 55(3): 225 - 76.
Harbone JB . Metode Fitokimia. Bandung: ITB; 1987 ; 49.
Tangkery RAB, Paransa DS, and Rumengan A . Test of Anticoagulant Activity Mangrove Extract Aegiceras c orniculatum. Journal of Coastal and Tropical Seas 2013; 1(1): 7 - 14.
Banfi G, Salvagno GL , and Lippi G . The Role o f Ethylenediamine Tetraacetic Acid (EDTA) a s In Vitro Anticoagulant f or Diagnostic Purposes. Clinical Chemistry and Laboratory Medicine: CCLM/FESCC 2007 ; 45: 565 – 576.
Seminoff JA, Lemons GE, Eguchi T, Lyon BN, and Leroux R . Effects of blood anticoagulants on stable isotope values of sea turtle blood tissue. Aquatic B iology 2012 ; 14: 201 – 206
Nowack B, Kari FG , and Krüger HG . The Remobilization of Metals From Iron Oxides And Sediments By Metal - Edta Complexes. Water, Air, and Soil Pollution 2001 ; 125: 243 – 257
Anand M , Rajagopal K , and Rajagopal KR . A Model f or The Formation And Lysis Of Blood Clots . Pathophysiol Haemost Thromb 2005; 34(2 - 3):109 - 20.
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