Biosynthesis and evaluation of TiO2 and ZnO nanoparticles from in vitro stimulation of Lactobacillus johnsonii

Authors

  • Hind A. Al-Zahrani Biology Dept., Faculty of Science, University of Jeddah, Saudi Arabia
  • Amr A. El-Waseif Botany and Microbiology Dept., Faculty of Science (Boys), Al-Azhar University, Cairo, Egypt
  • Dina E. El-Ghwas Biology Dept., Faculty of Science, University of Jeddah, Saudi Arabia. Chemistry of Natural and Microbial products Dept., National Research Center, Dokki, Egypt.

DOI:

https://doi.org/10.56511/JIPBS.2018.v5.i01.pp16-20

Abstract

The microbial synthesis of nanoparticles is advantageous more other chemical and physical methods. Biosynthesis of TiO2 and ZnO Nanoparticles (NPs) were carried out using Lactobacillus johnsonii that isolated from human gut and identified biochemical in previous study. Results of molecular identification using 16S DNA of Lactobacillus strain revealed that similarity in phylogenic tree is 100% with Lactobacillus johnsonii. Synthesis in MRS broth and evaluation of TiO2 and ZnO nanoparticles from Lactobacillus johnsonii carried out using UV, FTIR, and TEM. Charts results of UV experiment for both nanoparticles indicated that absorbance at 409 and 492 nm is typically for TiO2 and ZnO Nanoparticles respectively. Also, FTIR peaks of TiO2 chart has confirmed that the stronger ability of proteins to bind metal, and grow the possibility of coating the metal nanoparticles with proteins to prevent agglomeration of the particles. TEM images at different magnification explain the shape and size range of TiO2 and ZnO Nanoparticles. TEM of TiO2 NPs recorded irregular shapes but, ZnO NPs spherical, results shows also, average size between 4-9 nm in both cases

Keywords:

TiO2 and ZnO Nanoparticles, UV, FTIR, TEM and Lactobacillus

Downloads

Download data is not yet available.

References

Tripathy, A., A., Raichur, M., Chandrasekaran, N., Prathna, T.C. and Mukherjee, A: Process variables in biomimetic synthesis of silver nanoparticles by aqueous extract of Azadirachtaindica (Neem) leaves. Journal of Nanoparticle Research 2010; 12 (1): 237-246.

Malarkodi,C., Chitra,K. Rajeshkumar, K., Gnanajobitha, K., Paulkumar, M., Vanaja, G. and Annadurai,G: Novel eco-friendly synthesis of titanium oxide nanoparticles by using Planomicrobium sp. and its antimicrobial evaluation. Der Pharmacia Sinica 2013; 4(3):59-66.

Popecu,M., Velea, A. and Lorinczi, A: Biogenic production of nanoparticles. Digest Journal of Nanomaterials and Biostructures 2010; 5(4): 1035 – 1040.

Parashar U.K., Saxena P.S., and Srivastava A.: Bioinspired Synthesis of Silver Nanoparticles. Digest J. Nanomater. Biostruct 2009; 4(1): 159-166.

Goldie O., Sunil P., Ashmi M. and Madhuri S.: Extracellular Biosynthesis of Gold Nanoparticles Using Salmonella typhyi. Der Chemica Sinica 2012; 3(5):1041-1046.

Prakash, A., Sharma, S., Ahmad , N., Ghosh, A. and Sinha, P.: Bacteria mediated extracellular synthesis of metallic nanoparticles. International Research Journal of Biotechnology 2010; 1(5): 071-079.

Sagadevan,S.: Synthesis and electrical properties of TiO2 nanoparticles using a wet chemical technique. American Journal of Nanoscience and Nanotechnology 2013; 1(1): 27-30.

Park, S., Lee, J.H., Kim, H.S., Park, H.J., Lee, J. C.: Effects of ZnO nanopowder dispersion on photocatalytic reactions for the removal of Ag ions from aqueous solution. J. Electroceram 2009; 22(1): 105 – 109.

Gong X.Q., and Selloni A.: Reactivity of Anatase TiO2 Nanoparticles. The Role of the Minority (001) Surface Surface. J. Phys. Chem. 2005; 109(42): 19560-19562.

Jha A.K., Prasad K., and Kulkarni A.R.: Synthesis of TiO2 Nanoparticles Using Microorganisms. Colloids and Surfaces B: Biointerfaces 2009; 71(2): 226 – 229.

Pan X., Ramirez I.M., Mernaugh R., Mernaughc R. and Liu J.: Nanocharacterization and bactericidal performance of silver modified titania photocatalyst. Colloids and Surfaces B: Biointerfaces 2010; 77(1):82 – 89.

Prasad K., Jha A.K., and Kulkarni A.R.: Lactobacillus assisted synthesis of Tritium Nanoparticles. Nanoscale Res Lett. 2007; 2(1): 248 – 250.

Jha, A.K, Prasad , K. and Kulkarni, A.R: Synthesis of TiO2 nanoparticles using microorganisms. Colloids Surf B Biointerfaces 2009; 71 (2):226 – 229.

De Man, J.C., M. Rogosa and E. Sharpe: A medium for the cultivation of Lactobacilli. J. Appl. Bacteriol. 1960; 23(1):130-135.

Ventura, M., M. Elli, R. Reniero and R. Zink: Molecular microbial analysis of bifidobacterium isolates from different environments by the species-specific amplified ribosomal DNA restriction analysis (ARDRA). FEMS Microbiol. Ecol. 2001; 36(1): 113 – 121.

Azhar,A.M., Behtas Ladan, S.A., Ajabadi Ebrahimi, M.T. and Heydari,M.: Lactobacillus-mediated biosynthesis of titanium nanoparticles in MRS broth medium . Brno, Czech Republic, EU. 2011; 9(1): 21 – 23.

Malarkodi C., Chitra K., Rajeshkumar S., Gnanajobitha G., Paulkumar K., Vanaja M. and Annadurai G.: Novel eco-friendly synthesis of titanium oxide nanoparticles by using Planomicrobium sp. and its antimicrobial evaluation. Pelagia Research Library 2013; 4(3):59-66.

Selvarajan E., and Mohanasrinivasan V.: Biosynthesis and characterization of ZnO nanoparticles using Lactobacillus plantarum VITES07. Materials Letters 2013; 112(1): 180-182.

Published

05-01-2018
Statistics
282 Views | 181 Downloads

How to Cite

Hind A. Al-Zahrani, Amr A. El-Waseif, and Dina E. El-Ghwas. “Biosynthesis and Evaluation of TiO2 and ZnO Nanoparticles from in Vitro Stimulation of Lactobacillus Johnsonii”. Journal of Innovations in Pharmaceutical and Biological Sciences, vol. 5, no. 1, Jan. 2018, pp. 16-20, doi:10.56511/JIPBS.2018.v5.i01.pp16-20.

Issue

Volume 5, Issue 1, 2018 (Jan-Mar)

Section

Research Article

Copyright (c) 2018 Hind A. Al-Zahrani, Amr A. El-Waseif, Dina E. El-Ghwas

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.