Isolation of indole alkaloids from Kopsia larutensis King & Gamble and their effects on histamine and β-hexosaminidase inhibitory in RBL-2H3 cell line

Authors

  • Muhammad Syafiq Shahari Drug & Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz 50300, Kuala Lumpur, Malaysia
  • Anisah Fathin Ismail Drug & Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz 50300, Kuala Lumpur, Malaysia
  • Endang Kumolosasi Drug & Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz 50300, Kuala Lumpur, Malaysia
  • Nor Fadilah Rajab Biomedical Science Programme, Faculty of Health Science, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz 50300, Kuala Lumpur, Malaysia
  • Khairana Husain Drug & Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz 50300, Kuala Lumpur, Malaysia

Abstract

This present study was aimed to isolate the indole alkaloids from various parts of K. larutensis King & Gamble. The indole alkaloids were isolate and identify by various chromatography and spectroscopic techniques. The major isolated compounds were tested for anti-allergic activity by evaluation of histamine and β-hexosaminidase release inhibitory activities in RBL-2H3 cells. The extracts of K. larutensis stem bark and leaves gave a new compound identified as kopsilarutensinine (1), two known compounds namely (-)-eburnamine (2) and kopsinine (3), and tetrahydroalstonine (4) which first time being reported in this plant. Crude alkaloids of stem bark and leaves showed highest inhibitory activities as compared to other parts. Meanwhile, compound 4 exhibited the highest inhibitory significantly among the isolates. These results were supported by docking simulation study which showed binding of compound 4 with responsible key residues. In conclusions, indole alkaloids from K. larutensis have potential as alternative anti-allergic agent.

Keywords:

Kopsia larutensis, Indole alkaloids, Histamine, β-hexosaminidase, Anti-allergic activity, RBL-2H3 cells

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Published

05-07-2017
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How to Cite

Muhammad Syafiq Shahari, Anisah Fathin Ismail, Endang Kumolosasi, Nor Fadilah Rajab, and Khairana Husain. “Isolation of Indole Alkaloids from Kopsia Larutensis King & Gamble and Their Effects on Histamine and β-Hexosaminidase Inhibitory in RBL-2H3 Cell Line”. Journal of Innovations in Pharmaceutical and Biological Sciences, vol. 4, no. 3, July 2017, pp. 80-86, https://jipbs.com/index.php/journal/article/view/241.

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Research Article