LC/MS, GC/MS screening and in vivo anti-inflammatory activity of Malaysian Moringa oleifera Lam leaf extracts and fractions against carrageenan -induced paw oedema in rats

Authors

  • Harith Jameel Mahdi Discipline of Pharmaceutical Technology, School of pharmaceutical Sciences, USM, Malaysia
  • Nurzalina Abdul Karim Khan Discipline of Pharmaceutical Technology, School of pharmaceutical Sciences, USM, Malaysia
  • Roziahanim Mahmud Discipline of Pharmaceutical Chemistry, School of pharmaceutical Sciences, USM, Penang, Malaysia
  • Mohd Zaini Bin Asmawi Discipline of Pharmacology, School of pharmaceutical Sciences, USM, Penang, Malaysia
  • Vikneswaran A/L Murugaiyah Discipline of Pharmacology, School of pharmaceutical Sciences, USM, Penang, Malaysia

Abstract

 

The anti-inflammatory activity of three extracts of Moringa leaf and the fractions of most active extracts was evaluated. Carrageenan-induced paw oedema in rats was used to evaluate the anti-inflammatory activity and to determine the effective dose of M. oleifera leaf extract and its fractions. In addition to that, LC/MS and GC/MS analysis of most active fraction were used to identify the phytoconstituents. 95% ethanol extract, at dose of 250 mg/kg body weight in rat, and its dichloromethane fraction found to be the most active as anti-inflammatory. LC/MS identified 18 compounds and GC/MS identified 8 compounds including flavinoids, phenol glucosides, amino acids and vitamin. A more detailed studies, including in human studies, to identify the phytochemical(s) and to establish the mechanism of action responsible for  anti-inflammatory activity are highly recommended

Keywords:

Carrageenan, Moringa oleifera, Anti-inflammatory, LC/MS and GC/MS

Downloads

Download data is not yet available.

Published

05-07-2017
Statistics
43 Views | 22 Downloads

How to Cite

Harith Jameel Mahdi, N. A. K. Khan, Roziahanim Mahmud, M. Z. B. Asmawi, and Vikneswaran A/L Murugaiyah. “LC/MS, GC/MS Screening and in Vivo Anti-Inflammatory Activity of Malaysian Moringa Oleifera Lam Leaf Extracts and Fractions Against Carrageenan -Induced Paw Oedema in Rats”. Journal of Innovations in Pharmaceutical and Biological Sciences, vol. 4, no. 3, July 2017, pp. 48-54, https://jipbs.com/index.php/journal/article/view/236.

Issue

Section

Research Article