Application of ISSR analysis for identification of lactic acid bacteria strains isolated from Egyptians milk products
DOI:
https://doi.org/10.56511/JIPBS.2016.v3.i01.pp204-213Abstract
The aim of this study is to isolate and identify probiotic lactic acid bacteria. About four isolates of lactic acid bacteria were isolated from different sources of milk products. Identification of isolates was done by morphological, biochemical and physiological characterizations. Results indicated that all the four isolates were able to tolerate acidic conditions at low pH (3) and survived at different concentration of bile salts (0.5-1.5%) and showed tolerance to 6.5% NaCl concentration. All isolates have antioxidant activity ranged from 63.2% to 83.7% and cholesterol removal ranged from 17% to 27%. The genetic relationships among the isolates were determined by Inter Simple Sequence Repeat (ISSR) technique Polymerase Chain Reaction (PCR), the results demonstrated a distinctive comparison of clusters and their pattern was greatly related to the clustering obtained with the morphological and biochemical identification. The selected lactic acid bacteria exhibited excellent probiotic characteristics and thus can be used as a potential source of probiotic.
Keywords:
probiotic, ISSR, β-galactosidase, antioxidantDownloads
References
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