Effect of Acid Base on different Brands of Sitagliptin by using UV Spectrophotometer

Abstract

The main objective of this study is to determine the effect of acidic and basic medium on different brands of Sitagliptin. It is usually preferred over other methods because of less equipment cost and economical maintenance advantage. For this reason we prepared a 200ppm solution of different brands of Sitagliptin (Sita, Trevia, Tagip). To determine the effect of acid and base on different brands of Sitagliptin (Sita, Trevia, Tagip) the 200 ppm solution of each brand was transferred in to two separate test tubes that contain 5ml of acid HCl and base NaOH separately. Then the tubes were left for 30 minutes. The absorbance of the solution was determined using spectrophotometer at wavelength max 266nm. After taking the absorbance of different brands .The result reveals that the percent availability of different brands if sitagliptin in acidic and basic media was found to be increase which shows that it contains the degradeable components which are responsible for increase in absorbance in uv region. We conclude that the drug shows degradation in both the mediums.