Effect of ethanolic extract and n-hexane fraction of Moringa oleifera L. against cell cycle and apoptosis MCF-7 cell lines
DOI:
https://doi.org/10.56511/JIPBS.2019.v6.i01.pp25-30Abstract
Objective: The aim of this study was to determine the effect of ethanolic extract (EE) and n-Hexane fraction (NHF) of Moringa oleifera L. leaf on cytotoxic effect, cell cycle and apoptosis on MCF-7 cell lines. Methods: In vitro Cytotoxic assays was determined by MTT (Microculture Tetrazolium Tehnique) assay, cell cycle inhibition and apoptosis were determined with flow cytometry. Results: Cytotoxic activity of EE with IC50 94.44 µg / mL and 97.60 µg / mL value for NHF. The samples were caused cell accumulation in the G0-G1 phase and late necrotic region for apoptosis effect. Conclusion: EE and NHF are potentially to be developed as chemotherapeutic agent for breast cancer, while molecular mechanism needs to be explored.
Keywords:
Moringa oleifera, Cell Cycle, Apoptosis, MCF-7 cell linesDownloads
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