Effect of ethanolic extract and n-hexane fraction of Moringa oleifera L. against cell cycle and apoptosis MCF-7 cell lines

Authors

  • Masfria Department of Pharmacology, Faculty of Pharmacy, University of Sumatera Utara, Medan, Indonesia
  • Poppy Anjelisa Zaitun Hasibuan Department of Pharmacology, Faculty of Pharmacy, University of Sumatera Utara, Medan, Indonesia
  • Azizah Daulay Department of Pharmacology, Faculty of Pharmacy, University of Sumatera Utara, Medan, Indonesia

Abstract

Objective: The aim of this study was to determine the effect of ethanolic extract (EE) and n-Hexane fraction (NHF) of Moringa oleifera L. leaf on cytotoxic effect, cell cycle and apoptosis on MCF-7 cell lines. Methods: In vitro Cytotoxic assays was determined by MTT (Microculture Tetrazolium Tehnique) assay, cell cycle inhibition and apoptosis were determined with flow cytometry. Results: Cytotoxic activity of EE with IC50  94.44 µg / mL and 97.60 µg / mL value for NHF. The samples were caused cell accumulation in the G0-G1 phase and late necrotic region for apoptosis effect. Conclusion: EE and NHF are potentially to be developed as chemotherapeutic agent for breast cancer, while molecular mechanism needs to be explored.

 

Keywords:

Moringa oleifera, Cell Cycle, Apoptosis, MCF-7 cell lines

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Published

05-01-2019
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How to Cite

Masfria, P. A. Z. Hasibuan, and Azizah Daulay. “Effect of Ethanolic Extract and N-Hexane Fraction of Moringa Oleifera L. Against Cell Cycle and Apoptosis MCF-7 Cell Lines”. Journal of Innovations in Pharmaceutical and Biological Sciences, vol. 6, no. 1, Jan. 2019, pp. 25-30, https://jipbs.com/index.php/journal/article/view/356.

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Research Article