Antibacterial effect of photo-activated zinc oxide nanoparticles capped with different polymers
DOI:
https://doi.org/10.56511/JIPBS.2018.v5.i04.pp111-119Abstract
In this paper, three types of zinc oxide nanoparticles (ZnO NPs) were prepared by a wet chemical method (precipitation method), the first type was ZnO NPs capped with Polyethylene Glycol (ZnONPs@PEG) and other ZnO NPs were capped with Polyvinylpyrroli-done (ZnO NPs@PVP), and the last type of ZnO NPs was provided without polymer. The samples were characterized via X-ray diffraction (XRD). The average crystal size and shape of the prepared ZnO nanopowder were determined by Transmission Electron Microscope (TEM). The antibacterial activity of the three types of ZnO NPs were tested against four types of bacteria that were Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus, All ZnO NPs were photo-activated under the exposure to UV light at 254 nm before being applied against the four types of bacteria. The antibacterial activity of ZnO NPs was determined based on the appearance of zones of inhibition and the standard deviation was calculated for all appeared inhibition zones. The results obtained from TEM imaging revealed the formation of spherical, rods and nano flowers shapes for uncapped ZnO NPs, ZnONPs@PEG and ZnO NPs@PVP respectively. Also, it was found that the Bacillus subtilis inhabited using ZnONPs@PEG more than using the other types of ZnO NP
Keywords:
ZnO nanoparticles, PVP, X-ray diffraction, TEM, BacillusDownloads
References
Akhavan O,Ghaderi, E. Enhancement of antibacterial properties of Ag nanorods by electric field. Sci. Technol. Adv. Mater 2009; 10(1): 1 - 5.
Roselli M, Finamore, A, Garaguso, I, Britti, MS, Mengheri, E. Zinc oxide protects cultured enterocytes from the damage induced by Escherichia coli. J. Nutr. 2003; 133(12): 4077-4082.
He Y, Chen, C-Y. Quantitative analysis of viable, stressed and dead cells of Campylobacter jejuni strain 81-176. Food Microbiol. 2010; 27(4): 439-446.
Maier B, Potter, L, So, M, Seifert, HS, Sheetz, MP. Single pilus motor forces exceed 100 pN. Proc. Natl. Acad. Sci. 2002; 99(25): 16012-16017.
Boating JS. Analysis of commercial samples of aloe. 2000; Ph.d., University of Strathclyde., Glasgow, United Kingdom.
Lin J, Qiu, S, Lewis, K, Klibanov, AM. Bactericidal properties of flat surfaces and nanoparticles derivatized with alkylated polyethylenimines. Biotechnol. Prog. 2002; 18(5): 1082-1086.
Gupta MK, Mishra, B, Prakash, D,Rai, SK. Nanoparticulate drug delivery system of cyclosporine. Int. j. pharm. pharm. sci. 2009; 1(2): 81-92.
Zhang L, Jiang, Y, Ding, Y, Povey, M, York, D. Investigation into the antibacterial behaviour of suspensions of ZnO nanoparticles (ZnO nanofluids). J. Nanopart Res. 2007; 9(3): 479-489.
Padmavathy N, Vijayaraghavan, R. Enhanced bioactivity of ZnO nanoparticles — an antimicrobial study. Sci Technol Adv Mater. 2008; 9(3): 035004.
Shah M, Al-Shahry, M. Zinc oxide nanoparticles prepared by the reaction of zinc metal with ethanol. Science 2009; 21(1):61-67.
Bauer A, Kirby, W, Sherris, JC,Turck, M. Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol. 1966; 45(4): 493.
Lowy FD. Staphylococcus aureus infections. N. Engl. J. Med. 1998; 339(8): 520-532.
Sawai J. Quantitative evaluation of antibacterial activities of metallic oxide powders (ZnO, MgO and CaO) by conductometric assay. J Microbiol Methods. 2003; 54(2): 177-182.
Kühn KP, Chaberny, IF, Massholder, K, Stickler, M, Benz, VW, Sonntag, H-G, Erdinger, L. Disinfection of surfaces by photocatalytic oxidation with titanium dioxide and UVA light. Chemosphere 2003; 53(1): 71-77.
Brayner R, Ferrari-Iliou, R, Brivois, N, Djediat, S, Benedetti, MF,Fiévet, F. Toxicological impact studies based on Escherichia coli bacteria in ultrafine ZnO nanoparticles colloidal medium. Nano letters 2006; 6(4): 866-870.
Gaballa A,Helmann, JD. Identification of a zinc-specific metalloregulatory protein, Zur, controlling zinc transport operons in Bacillus subtilis. J. Bacteriol. 1998; 180(22): 5815-5821.
Xiong A, Jayaswal, RK. Molecular Characterization of a Chromosomal Determinant Conferring Resistance to Zinc and Cobalt Ions in Staphylococcus aureus. J. Bacteriol. 1998; 180(16): 4024-4029.
Navarre WW, Schneewind, O. Surface proteins of gram-positive bacteria and mechanisms of their targeting to the cell wall envelope. Microbiol Mol Biol Rev. 1999; 63(1): 174-229.
Tiller JC, Liao, C-J, Lewis, K,Klibanov, AM. Designing surfaces that kill bacteria on contact. Proc. Natl. Acad. Sci. 2001; 98(11): 5981-5985.
Published
How to Cite
Issue
Section
Copyright (c) 2018 Ola M. El-Borady, Ali Diab, Doaa M. Al-Faqih, Ahmed F. El-Sayed
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Journal of Innovations in Pharmaceutical and Biological Sciences is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. Based on a work at