Inhibitory effect of ethanolic extract of Physalis minima L. (Ciplukan) leaves on acetylcholine muscarinic-3 receptors induced on isolated guinea pig tracheal

Abstract

Objective: The study was aimed to investigate the inhibitory effect of ethanolic extract of Physalis minima L. leaves (EEPML) against acetylcholine (ACh)-induced contraction of the ACh-muscarinic-3 receptor. Materials and methods: The study of the inhibitory effect of the ethanolic extract of physalis minima L. on the contraction by ACh concentration series (1x10⁻⁸-3x10⁻³ M) was conducted in vitro using isolated guinea pig tracheal organ in the Krebs solution. Results: EEPML (0.5 - 4 mg/ml) concentration has relaxation effect on the trachea smooth muscle contraction induced by acetylcholine 1.43 x 10^-4 M (r=0.982; p<0.05). EEPML has concentration of 4mg/ml, that is not different with atropine sulfate 1 x 10^-6 M in reduced the contraction of guinea pig’s trachea smooth muscle induced by acetylcholine 1.43 x 10^-4 M (p>0.05). EEPML at 0.5 - 4 mg/ml concentration has relaxation effect, EEPML 3.2 M has no difference in terms of ability as atropine sulfate 1 x 10^-6 M on the trachea smooth muscle contraction induced by ACh 1.43 x 10^-4 M. It showed that the possibility of the mechanism of the relaxation effect of EEDC mediated through inhibition muscarinic receptors Conclusion: The EEPML has relaxation effect on the trachea smooth muscle contraction induced by ACh And showed antagonist effect on ACh-muscarinic-3 receptor