UV Spectrophotometric Method Development and Validation for quantitative estimation of Nizatidine
A novel, safe and sensitive method of spectrophotometric estimation in UV-region has been developed for the assay of Nizatidine in tablet formulation. The method was developed are based on the solubility of Nizatidine in 0.1 N HCl (pH 1.2). The drug showed maximum absorbance (λmax) at 325 nm and linearity (Lambert Beer’s Range) was found in concentration range of 5-40 μg/ml and the standard curve equation was found to be y = 0.025x + 0.005 and R2 value 0.998. The results of analysis were validated statistically and by recovery studies. All the parameters of the analysis were chosen according to ICH [Q2(R1)] guidelines.