Comparing the effects of Sodium lauryl sulphate and Acacia concinna in dermatophagoides extract ointment induced atopic dermatitis

Abstract

Background: The obvious dermatologic symptoms, psychological stress, sleep problems, and poor social and occupational functioning that accompany an inflammatory skin condition known as atopic dermatitis (AD), substantially diminish the quality of life for those who suffer from it. Animal models, particularly those induced by relevant allergens like Dermatophagoides farinae (DNFB), are crucial for studying the pathophysiology and potential treatments for AD. Objective: This study aims to compare the effects of Sodium Lauryl Sulfate (SLS), a common ingredient in shampoos, with Acacia concinna, a natural alternative, on AD-like symptoms. In this study, we evaluate the effectiveness of these chemicals by applying DNFB extract ointment to a mouse model of atopic dermatitis, concentrating on clinical manifestations, skin histopathology, and immune responses. Methods: Along with disturbance of their skin's protective barrier, regular application of DNFB extract ointment to the backs of mice produced skin lesions similar to those seen in atopic dermatitis. Using clinical scoring, histological analysis, and inflammatory marker measurement, the degree of dermatitis was evaluated. The effects of SLS and Acacia concinna were compared to those of a corticosteroid treatment. Results: Dermatitis scores, which show symptoms including redness, swelling, erosion, and dry skin, were significantly elevated after multiple applications of DNFB extract ointment. Both the dermis and the epidermis thickened, according to the histology report, heightened transepidermal water loss, and higher levels of inflammatory cytokines, closely mirroring human atopic dermatitis. Treatment with Acacia concinna and corticosteroids effectively reduced these symptoms, suggesting potential therapeutic benefits. Conclusion: The DNFB-induced murine model of AD is a valuable tool for studying the disease and evaluating treatments. Acacia concinna shows promise as a safer alternative to SLS in managing AD, warranting further investigation.