Development and validation of high performance liquid chromatography methods (HPLC) analysis for simultaneous determination of Acetaminophen, Propyphenazone and Caffeine

Authors

  • IkaJulianti Tambunan Magister Program of Pharmacy, Faculty of Pharmacy, Universitas Sumatera Utara, Medan 20155, Indonesia
  • Effendy De Lux Putra Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan 20155, Indonesia
  • Siti Morin Sinaga Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan 20155, Indonesia

Abstract

Objective: The purpose of this research was to conduct an analysis with a high-performance liquid chromatography (HPLC) method for determining a simultaneously mixture of acetaminophen, propyphenazone and caffeine. Methods: This descriptive-experimental study was conducted by the HPLC method. This research was conducted by optimizing the ratio of the mobile phase of methanol: water (10:90), (20:80), (30:70), (40:60), (50:50), (60:40), (70: 30), (80:20), (90:10), flow rates 0.5-2.0 mL/min and column temperatures 30, 40, and 50°C. The analytical parameters in the validation test include accuracy, precision, limit of detection (LOD), limit of quantitation (LOQ). Results: The results showed that the mobile phase of methanol: water (70:30) was a good optimization result. The average results of determining the levels of acetaminophen, propyphenazone and caffeine in the preparation of Bodrex Migraine® were 101.35 ± 0.16%, 99.24 ± 0.14%, 97.95 ± 0.16%, and Saridon® were 99.50 ± 0.07%, 100.25 ± 0.12%, 98.94 ± 0.06%. The results of the validation parameters performed on acetaminophen, propyphenazone and caffeine were 99.66%, 98.89% and 100.24% for the average recovery results, and 0.01738, 0.01751, 0.02557 for precision, and 1.00026, 0.69104, 0.38920 for the average LOD results and 3.33423, 2.30347, 1.29735 for LOQ average results. Conclusion: The mobile phase of methanol: water (70:30) and the flow rate of 1 mL/min with a column temperature of 30°C was a good optimization result for use in the simultaneous analysis of a mixture of acetaminophen, propyphenazone and caffeine

Keywords:

HPLC, determination, acetaminophen, propyphenazone, caffeine

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Published

22-08-2020
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How to Cite

IkaJulianti Tambunan, Effendy De Lux Putra, and Siti Morin Sinaga. “Development and Validation of High Performance Liquid Chromatography Methods (HPLC) Analysis for Simultaneous Determination of Acetaminophen, Propyphenazone and Caffeine”. Journal of Innovations in Pharmaceutical and Biological Sciences, vol. 7, no. 3, Aug. 2020, pp. 64-71, https://jipbs.com/index.php/journal/article/view/395.

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Research Article