Antibacterial Activity of Spices against Enterobacteriaceae

Romita Marwaha; Pathan Azhar  Khan; Manadar Abhyankar

doi:10.56511/JIPBS.2015.v2.i01.pp34-44

Authors

Romita Marwaha Student, Department of Clinical Research, CRB Tech, Pune. India
Pathan Azhar Khan Trainer, Department of Clinical Research, CRB Tech, Pune, India
Manadar Abhyankar Director, CRB Tech, Pune. India

DOI:

https://doi.org/10.56511/JIPBS.2015.v2.i01.pp34-44

Abstract

Present research work deals with the Study of Antibacterial Activity of Spices against Enterobacteriaceae (E.coli). Aqueous solution of Spices (Bay leaf (Laurus nobilis), Black pepper (Pippali nigrum), Cinnamon (Cinamomum verum), Clove (Syzygium aromaticum) and Star Anise (Illicium verum) was prepared and tested against Enterobacteriaceae (E.coli). Antibacterial activity of Spices was tested on E.coli by Disc diffusion method and antibacterial activity of each spice was observed in the form of zone of inhibition. Black pepper (Pippali nigrum) showed 2cm antibacterial activity on E.coli as compared to Bay leaf (Laurus nobilis) 1.3cm and Star Anise (Illicium verum) 0.9cm. Cinnamon (Cinamomum verum) Clove (Syzygium aromaticum) and does not showed any antibacterial activity at the concentration of 10µl/disc. The results showed that the aqueous decoction of black pepper possesses great antibacterial potential (75%) as compared to aqueous decoction of bay leaf (53.4%) and star anise (18.1%).

Keywords:

Bacterial culture (E.coli), Spices [Bay leaf (Laurus nobilis), Black pepper (Pippali nigrum), Cinnamon (Cinamomum verum), Clove (Syzygium aromaticum), and Star Anise (Illicium verum), Agar Disc Diffusion Method

Downloads

Download data is not yet available.

References

Indian journal of Natural Products and Resources Vol. 1 (2), June 2010, pp. 213-

Antibacterial activity of black pepper(Piper nigrum Linn.) with special reference to its mode of action on bacteria Pavithra Vani Karsha O Bhagya Lakshmi Department of Microbiology and Department of Botany Sarojini Naidu Vanita Maha Vidhyalaya Exhibition ground, M J Road, Nampally, Hyderabad500 001 , Andhra Pradesh, India Received 8 June 2009; Accepted 7 December 2009

In Vitro Antibacterial Activity of Clove Against Gram Negative Bacteria Sabahat Saeed And Perween Tariq Department of Microbiology, University of Karachi, Karachi-75270, Pakistan Pak. J. Bot., 40(5): 2157-2160, 2008.

Bactericidal Activity of Black Pepper, Bay Leaf, Aniseed And Coriander Against Oral Isolates Nazia Masood Ahmed Chaudhry And Perween Tariq Department of Microbiology, University of Karachi, Karachi-75270, Pakistan Pak. J. Pharm. Sci., 2006, Vol. 19(3), 214-218.

Kmitl Sci. Tech. J. Vol. 5 No. 3 Jul. – Dec. 2005 antibacterial Activity of Crude Ethanolic Extracts And Essential Oils Of Spices Against Salmonella And Other Enterobacteria Suree Nanasombat and Pana lohasupathawee Department of Applied Biology , Faculty of Science, King Mongkut’s Institute of Technology Ladkrabang, Chalongkrung Road, Ladkrabang, Bangkok 10520, Thailand

Internet Journal of Food Safety, Vol.12, 2010, p. 71-75 Copyright© 2010, Food Safety Information Publishing Antimicrobial and Biochemical Analysis of Some Spices Extract against Food Spoilage Pathogens M. Syed Abdul Rahman, S. Thangaraj, S. Mohamed Salique, K. Feroz khan1 and S. Esath Natheer Centre of Advanced Study in Marine Biology, Annamalai University, Parangipettai - 608 502, Tamil Nadu, India. 2 The Head of the Department of Microbiology, Jamal Mohamed College, Bharathidasan University, Tiruchirappalli, - 620 020. Tamilnadu, India. 3 Department of Microbiology, Annamalai University, Annamalai Nagar608 002. Tamilnadu, India.

Isolation and Identification of Two Bacterial Unknowns (Theory) Microbiology Virtual Lab II Biotechnology and Biomedical Engineering Amrita Vishwa Vidyapeetham Virtual Lab.htm.

Experiments in Microbiology, Plant Pathology and Biotechnology, Fourth Edition K.R Aneja.

http://onlinelibrary.wiley.com/doi/10.1 111/j.1365-2621.2006.01308.x/abstract

Importance of Spices | eHow.com

http://www.ehow.com/about_5393428_ importance-spices.html#ixzz1xDeUlgP

http://www.ncbi.nlm.nih.gov/pmc/artic les/PMC3074903/

http://wiki.answers.com/Q/What_is_the _colony_morphology_for_escherichia_coli #ixzz25s6cGWOF

http://en.wikipedia.org/wiki/Agar_diffu sion_test

http://www.microbelibrary.org/library/ laboratory-test/2842-triple-sugar-ironagar-protocols

http://amrita.vlab.co.in/?sub=3&brch=7 3&sim=1139&cnt=2