UV spectrophotometric assay of Diclofenac sodium available brands

Abstract

Diclofenac is a nonsteroidal anti-inflammatory drug (NSAID). A efficient, simple and least time consuming spectrophotometric method for the assay of diclofenac sodium has been developed. Comparison of assay of four different brands of diclofenac sodium (Dicloran, Defnac, Artifin, voltral) has also been done available in public medical store of Karachi, Pakistan. The assay is based on the ultraviolet UV absorbance maxima at about 276nm wavelength of diclofenac sodium using water as solvent. A sample of drug was dissolved in water to produce a solution containing diclofenac sodium. Similarly, a sample of ground tablets of different brand were dissolved in water and various dilutions were made. The absorbance of sample preparation was measured at 276nm against the solvent blank and the assay was determined by comparing with the absorbance of available brand. The method can be applied for the routine QC quantitation of diclofenac sodium in tablet formulation and active. Our results reveals that among all the four brands of diclofenac sodium (Dicloran, Defnac, Artifin, voltral) Defnac and voltral shows highest percentage assay 104% and Dicloran shows lowest value for percentage assay 96.4% , while artifin shows a percentage assay of 98.6%.