Optimization of multiple valuable biogenic products using experimental design

Authors

  • Mostafa M. Abo Elsoud Microbial Biotechnology Dept., National Research Centre, Giza, Egypt
  • Heba I. Elkhouly Department of Botany and Microbiology, Faculty of Science, Al Azhar University (Girls Branch), Cairo, Egypt
  • Nagwa M. Sidkey Department of Botany and Microbiology, Faculty of Science, Al Azhar University (Girls Branch), Cairo, Egypt

Abstract

During the last few decades, biosurfactants (e.g. rhamnolipids) have received much more attention as an alternative to the conventional synthetic surfactants. The production cost of biosurfactant is the main hindrance for its commercialization. The production of multiple valuable products in a single process may contribute in reduction of the production cost. The scope of the current work was to use Plackett Burman design for selection of the most significant factors with highest contributionsfor production of rhamno lipids in parallel with protease using Pseudomonas aeruginosa (PAO1). Nineteen factors have been selected for this study. According to the obtained results, six obvious factors; glucose, casein, peptone, yeast extract, pH and NaCl, had to be added to the production medium and three others; urea, NaNO3, and (NH4)2SO4, should not. Some of the remaining factors were not significant and the others had contrary effects on rhamnolipids and protease production. The final results showed that, the use of factorial design resulted in 213.27% increase in rhamnolipid productivity and 296.65% increase in protease productivity compared with the original production conditions.

Keywords:

Biosurfactants, Pseudomonas aeruginosa (PAO1), Plackett Burman, rhamnolipid, protease

Downloads

Download data is not yet available.

Published

05-10-2017
Statistics
37 Views | 27 Downloads

How to Cite

Abo Elsoud, M. M., Heba I. Elkhouly, and Nagwa M. Sidkey. “Optimization of Multiple Valuable Biogenic Products Using Experimental Design”. Journal of Innovations in Pharmaceutical and Biological Sciences, vol. 4, no. 4, Oct. 2017, pp. 32-38, https://jipbs.com/index.php/journal/article/view/254.

Issue

Section

Research Article