Isolation and characterization of cellulolytic and hemicellulolytic fungi from salt affected soils and compost


  • Mohamed F. Eida Agricultural Microbiology Department, National Research Center, Giza, Egypt
  • Ibrahim A. Matter Agricultural Microbiology Department, National Research Center, Giza, Egypt
  • Fatma H. Abd El Zaher Agricultural Microbiology Department, National Research Center, Giza, Egypt


Lignocellulosic materials are hugely abundant agricultural biomass, which their bioconversion occurs by various microorganisms. Environmental conditions greatly affect the biological processes and the diversity of living organisms. The aim of this study was to isolate and characterize cellulolytic and hemicellulolytic  fungi and clearlying thier diversity and efficiency in salt affected soils and compost. Soil samples were collected from saline soils and municipal solid waste (MSW) compost. Cellulolytic fungi were isolated, then purified to single isolate. The isolates were identified morphologically. Cellulase, xylanase, glucanase, and mannanase were determined in plate assays using AZCL-substrates, while CMCase and FPase were evaluated by DNS method. Sixteen fungal isolates were directly isolated. The morphological identification of isolated fungi revealed Aspergillums and Penicillium genera were the dominant genera in saline environments. All the selected isolates exhibited cellulase and xylanase activity regardless their origin of isolation. Whereas some of Aspergillus isolated revealed ß-Glucanase activity, 56 % of isolates the produced mannanase.  The mannanase activities among Aspergillus sp. isolates were based on isolate as the production depended on the isolated itself irrespective to the species but may be related to isolation source. All isolated fungi have been confirmed to produce CMCase and FPase. The highest CMCase and FPase activity was displayed by A. glaucus, SS6 and Com3 isolated from Sinai saline soil in Gilbana and MSW compost, respectively. Some isolates showed high efficiency for bioconversion of lignocellulosic material, according their enzymatic system, and could mitigate salt and alkalinity stress which suggesting an importance in biotechnological applications


cellulolytic and hemicellulolytic fungi, Saline soils, MSW, enzyme assay


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Mohamed F. Eida, Ibrahim A. Matter, and F. H. Abd El Zaher. “Isolation and Characterization of Cellulolytic and Hemicellulolytic Fungi from Salt Affected Soils and Compost”. Journal of Innovations in Pharmaceutical and Biological Sciences, vol. 3, no. 4, Oct. 2016, pp. 164-70,



Research Article