Isolation of endophytic bacteria from plant basil (Ocimum sanctum L.) as antibacterials against staphylococcus aureus

Abstract

Background: Endophytic bacteria are microorganisms that live inside plant tissues such as the basil. The endophytic bacteria could be used as a source of drug compounds by utilizing secondary metabolites from bacteria such as bacteriocin. The purpose of this study was to isolate and test the antibacterial activity of secondary metabolites of endophytic bacteria. Materials and Methods: Methods used include the isolation of endophytic bacteria, identification of morphological (macroscopic and microscopic) and biochemistry, the determination of the growth curve, the extraction of secondary metabolites in the stationary phase, antibacterial activity test, test antibacterial properties, and screening of compounds active secondary metabolites of endophytic bacteria. Results: Isolation of endophytic bacteria from leaves of basil, obtained two strain. Based on the results of morphological and biochemical identification, these isolates belong to the genus Enterobacter. The results of the screening of secondary metabolites of endophytic bacteria in the stationary phase the best results were obtained at the 26th hour with a diameter of 6.05 mm inhibition. Secondary metabolites found to have antibacterial activity against Staphylococcus aureus with MIC value of 256 ppm. Conclusion: Endophytic bacteria isolated from the basil plant and have antibacterial activity against Staphylococcus aureus.