Synthesis, and in vitro antioxidant activity of novel 1, 3, 4- oxadiazole-2-thione

Abstract

As a continuation of our efforts to discover and develop 1,3,4-oxadiazole-2-thione,a new series of 5-[4-4′-nitrophenyl)-1,3-thiazole-2yl]-1,3,4-oxadiazole-2-thione (E) were synthesized and  derivatized in to mannich bases 5-[4-4′-nitro thiazole-2yl]-3-substituted-1,3,4-oxadiazole-2-thione [Ea-e]. Physical characterization of compounds which were established by performing melting point, R_f value, percentage yield and solubility. The derivative structures were identified using UV, FT-IR, ¹HNMR and MASS spectral analysis. The derivatives were then screened for antioxidant activity by DPPH, Nitric oxide and Hydrogen peroxide scavenging assays. When compared with other derivatives, compound 5c was found to have maximum in vitro antioxidant activity in all the three methods. This may be due to the presence of electron donating diethyl group present in compound ‘Ec’. The electron donating nitro group may also have an influence in the antioxidant activity which is present in all derivatives.