Assessment of odontogenic potential of mesenchymal stem cells derived from adipose tissue and oral mucosal tissue (comparative study)
DOI:
https://doi.org/10.56511/JIPBS.2016.v3.i02.pp182-192Abstract
Stem cell research has reached new heights in recent times and is implemented in regenerative medicine and dentistry. The oral cavity is the richest stem cell source in the human body. These stem cells are now recognized as being vital to different types of dental and non dental tissue regeneration. The aim of this study was to isolate human mesenchymal stem cells (MSCs) from Adipose tissue (ASCs) and oral mucosal tissue (OMSCs) and confirm their differentiation potentials, including the odontogenic lineage. ASCs and OMSCs cultures were nalyzed for cell shape, cell cycle, proliferation potential (MTT assay) and stem cell markers (CD90, CD105). The odontogenic differentiation potential of ASCs and OMSCs induced with odontogenic induction medium and was assessed by means of Alizarin Red stain and quantitative real time RT-PCR using dentin sialophosphprotein (DSPP). Our data revealed that ASCs and OMSCs showed a significant increase in cell viability from day 14 to day 21, representing high cell proliferation rate(80-90%), where ASCs proliferated faster than OMSCs. Moreover, ASCs and OMSCs can efficiently differentiate into dentin forming cells expressing odontoblastic markers (DSPP), where OMSCs significantly expressed (DSPP) higher than ASCs. This study provides evidence that ASCs and OMSCs can be used in tissue engineering/regeneration protocols as an approachable stem cell source.
Keywords:
stem cells, differentiation, odontogenic, adipose tissue, oral mucosaDownloads
References
Alfonso B. and Al-Rubeai M.: Flow cytometry. Compreh. Biotech. 2011; 1: 559578.
Angelova-Volponi A, Kawasaki M, Sharpe PT.: Adult human gingival epithelial cells as a source for whole-tooth bioengineering. J Dent Res 2013; 92:329-334.
Bong A., Eun J., June H., Myeong G., Hyun J., Young S., Tae H., Mohana K., Sun A. and Gyu J.: In vitro and in vivo osteogenesis of human mesenchymal stem cells derived from skin, bone marrow and dental follicle tissues. Differentiation 2012; 83: 249-259.
Bright R, Hynes K, Gronthos S, Bartold PM: Periodontal ligament-derived cells for periodontal regeneration in animal models: a systematic review. J Periodontal Res, 2014.
Chen F., Sun H., Lu H. and Yu Q.: Stem celldelivery therapeutics for periodontal tissue regeneration. Biomaterials 2012; 33: 63206344.
Davies O., Cooper P., Shelton R., Smith A. and Scheven A.: Comparison of the in vitro mineralization and dentinogenic potential of mesenchymal stem cells derived from adipose tissue, bone marrow and dental pulp. J. Bone Miner. Metab. 2014; 14: online springer.
Deepika N, Devadiga S , r Moidin T, Mittal S , Koshal N : Stem cells: A boon in dentistry, ournal of Dental Research and Review 2015; 2, 47-51.
Ekaterina I. and Vladimir P.: Photoacoustic flow cytometry. Methods 2012; 57: 280296.
Ensanya A., Wojciech C., Vehid M., Hae W. and Jonathan C.: Tissue engineering in dentistry. J. Dent. 2014; 15: 915-928.
Fawzy K., Dorfer C., Fandrich F., Gieseler F., Moustafa M. and Ungefroren H.: Adult mesenchymal stem cells explored in the dental field. Adv. Biochem. Eng. Biotechnol. 2012; 10: 151166.
Fournier BP, Larjava H, Häkkinen L: Gingiva as a source of stem cells with therapeutic potential. Stem Cells Dev 2013; 22: 3157-3177.
Ge S., Mrozik K., Menicanin D., Gronthos S. and Bartold P.: Isolation and characterization of mesenchymal stem celllike cells from healthy and inflamed gingival tissue: potential use for clinical therapy. Regen. Med. 2012; 7: 819-832.
Geetanjali B., Rupesh K., Navita G., Amruta P., Satish T., Hiral M., Gyan C. and Mohan R.: Human gingiva-derived mesenchymal stem cells are superior to bone marrowderived mesenchymal stem cells for cell therapy in regenerative medicine. Biochem. Biophys. Res. Comm. 2010; 393: 377-383.
Gronthos S., Batouli, S. Miura, M. and Brahim, J.: Comparaison between stem cell based osteogenesis and dentinogenesis. J. Dent. Res. 2003; 82: 976-981.
Hakan O., Morikuni T. and Hiroshi M.: Mesenchymal stem cells isolated from adipose and other tissues: basic biological properties and clinical applications. Stem Cells Int. 2012; 10: 10111020.
Indumathi S., Rashmi M., Harikrishnan R., Rajkumar S., Venkata N. and Dhanasekaran M.: Human adult stem cells maintain a constant phenotype profile irrespective of their origin, basal media and long term cultures. Stem Cells Inter. 2015; 4: 1-29.
Jin SH, Lee JE, Yun JH, Kim I, Ko Y, et al.: Isolation and characterization of human mesenchymal stem cells from gingival connective tissue. J Periodontal Res, 2014; 17.
Jones KB, Klein OD: Oral epithelial stem cells in tissue maintenance and disease: the first steps in a long journey. Int J Oral Sci. 2013; 5: 121-129.
Kabir, R., Gupta, M., Aggarwal, A., Sharma, D., Sarin, A., and Kola, M. Z.: Imperative role of dental pulp stem cells in regenerative therapies: a systematic review. Niger. J. Surg . 2014; 20.
Karim M., Sebastian P., Stephan B., Neemat K., Hendrick U., Fred F., Jorg W. and Christ of D.: Isolation and characterization of multipotent postnatal stem/progenitor cells from human alveolar bone proper. J. Cran. Max. Fac. Surg. 2012; 40: 735-742.
Keishi Otsu K , Sakano M K, Fujiwara N, Kikuchi K , Keller L, Lesot H, and Harada H: Stem cell sources for tooth regeneration: current status and future prospects. Front Physiol. 2014; 5: 36.
Kerkis I. and Caplan A.: Stem cells in dental pulp of deciduous teeth. Tissue Eng. 2012; 18: 129-138.
Lee A., Jienny L., Chan L., Keum S., So H., Na Y., Jeong M., Byeong C., Ok J., Jae Y. and Sang H.: Comparative studies on proliferation, molecular markers and differentiation potential of mesenchymal stem cells from various tissues (adipose, bone marrow, ear skin, abdominal skin and lung) and maintenance of multipotency during serial passages in miniature pig. Res. Vet. Sci. 2015; 1: 1-10.
Marcella L., Francesca P., Anna S., Pasqualina N., Roberta M., Vincenzo D., Alfredo D., Gianpaolo P., Virginia T. and Luigi L.: Dental pulp stem cells: state of the art and suggestions for a true translation of research into therapy. J. Dent. 2014; 2: 761768.
Marco T., Massimo M. and Francesco P.: The regenerative medicine in oral and maxillofacial surgery: the most important innovations in the clinical application of mesenchymal stem cells. Int. J. Med. Sci. 2015; 12: 72-77.
Martin G., Werner G., Oliver F., Tobias E., Andreas J. and Christian M.: Comparison of the differentiation potential of neural crest derived progenitor cells from apical papilla (dNC-PCs) and stem cells from exfoliated deciduous teeth (SHED) into mineralizing cells. Arch. Oral Biol. 2013; 58: 699-706.
Min S., Kyung G., Jaeseon L., Hyongbum K. and Seung H.: The effect of mineral trioxide aggregate on odontogenic differentiation in dental pulp stem cells. Basic Res. Biol. 2013; 39: 27-32.
Mizuno H., Tobita M. and Uysal C.: Concise review: adipose-derived stem cells as a novel tool for future regenerative medicine. Stem Cells 2012; 30: 804-810.
Mohamadreza B., Sima B. and Hamid N.: Odontogenic differentiation of dental pulp derived stem cells on tricalcium phosphate scaffolds. J. Dent. Sci. 2013; 8: 306-313.
Nguyen T., Mui B., Mehrabzadeh M., Chea Y., Chaudhry Z. and Chaudhry K.: Regeneration of tissues of the oral complex: current clinical trends and research advances. J. Canadian Dent. Assoc. 2013; 79.
Potdar, P. D., and Jethmalani, Y. D.: Human dental pulp stem cells: applications in future regenerative medicine. World J. Stem Cells 2015; 7:839.
Park Y J., Cha S., and Park Y S.: Regenerative Applications Using Tooth Derived Stem Cells in Other Than Tooth Regeneration: A Literature Review. Stem Cells International 2016; 2016.
Rouabhia M: Advantages and Limitations of Oral Stem Cell use for Oral Tissue Replacement. J Oral Bio. 2015; 2 (1): 9.
Seo M., Kyung-Gyun H., Hyongbum K. and Seung-Ho B.: Analysis of gene expression during odontogenic differentiation of cultured human dental pulp cells. Restor. Dent. Endod. 2012; 37: 142-148.
Shigeki S., Naoto H., Fusanori N. and Ashok B.: Dentin sialophosphoprotein and dentin matrix protein-1: two highly phosphorylated proteins in mineralized tissues. Arch. Oral Biol. 2012; 57: 11651175.
Teti G, Salvatore V, Focaroli S, Durante S, Mazzotti A, Dicarlo M, Belmonte M and Orsini G: In vitro osteogenic and odontogenic differentiation of human dental pulp stem cells seeded on carboxymethyl cellulose-hydroxyapatite hybrid hydrogel. Front. Physiol. 2015; 27.
Stephens P. and Davies L.: Oral mucosal progenitor cells stem cell biology and tissue engineering in dental sciences. PART | IV Oral and Craniofacial Stem Cells for Tissue Engineering. 2015 Elsevie r http://dx.doi.org/10.1016/B97 8-0-12397157-9.00025-4.
Verma, K., Bains, R., Bains, V. K., Rawtiya, M., Loomba, K., and Srivastava, S. C.: Therapeutic potential of dental pulp stem cells in regenerative medicine: an overview. Dent. Res. J. 2014; 11:302 – 308,.
Tatullo M, Marrelli M, Shakesheff KM, White LJ: Dental pulp stem cells: function, isolation and applications in regenerative medicine. J Tissue Eng Regen Med 2014, [Epub ahead of print].
Torkzaban P., Saffarpour A., Bidgoli M. and Sohilifar S.: In vitro evaluation of isolation possibility of stem cells from intra oral soft tissue and comparison of them with bone marrow stem cells. J. Dent. 2012; 9: 143156.
Vinicius R.: What and where are the stem cells for dentistry. Singapore Den. J. 2013; 34: 13-18.
Zuk P., Zhu M. and Mizuno H.: Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng. 2001; 7: 211-217.
Yu X, Ge S, Chen S, Xu Q, Zhang J, et al.: Human gingiva-derived mesenchymal stromal cells contribute to periodontal regeneration in beagle dogs. Cells Tissues Organs, 2013; 198: 428-437.
Zhang Q., Nguyen A., Yu W. and Le A.: Human oral mucosa and gingiva: a unique reservoir for mesenchymal stem cells. J. Dent. Res. 2012; 1: 91101.
Zhang Q., Shi S., Liu Y., Uyanne J., Shi Y. and Le A.: Mesenchymal stem cells derived from human gingiva are capable of immunomodulatory functions and ameliorate inflammation-related tissue destruction in experimental colitis. J. Immunol. 2009; 183: 7787-7798.
Published
How to Cite
Issue
Section
Copyright (c) 2016 Mohamad S. Ayoub, Effat A. Abbas, Houry M. Baghdadi, Reham AA Morsy, Wael Y. Elias, Dina S. Abd El Fattah, Heba E. Tarek
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Journal of Innovations in Pharmaceutical and Biological Sciences is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. Based on a work at