Freezability of buffalo semen by using tris extender supplemented with different concentrations of LDL


  • Shahba, M.I. Animal Reproduction and AI dept., Veterinary Researches Division, NRC, Dokki, Giza, Egypt
  • El-Sheshtawy, R.I. Animal Reproduction and AI, Veterinary Research Division, National Research Centre, Dokki, Egypt
  • El-Azab, A.I. Theriogenology dept., Fac.Vet.Med., Benha University, Egypt


The present study aimed at displaying the effect of LDL, as a cryopreservative, on the quality of the frozen-thawed buffalo semen. For buffalo semen extension, Tris-Citric acid-Fructose-Egg yolk extender was used as a control. Instead of Egg yolk, LDL fraction extracted from the Egg yolk would be added to the extender at6, 8, 10, and 12 % providing a concentration of 60 million sperm/ml. From the obtained results, the best semen quality was observed with the addition of 12% LDL to the frozen semen extender, as expressed by the highest sperm motility (32.50±1.50 %), sperm livability (72.00± 1.82 %), sperm cell membrane (69.10 ±1.84 %) and DNA (98.90 ±0.91 %) integrities as well as the lowest sperm abnormalities (09.10 ±1.48 %).  From the present study, the use of LDL at a concentration of 12% in buffalo frozen semen extender might be recommended to have better sperm motility, sperm livability and sperm abnormalities as well as sperm cell membrane and DNA integrities.


Buffalo, Semen, Freezability, Extender, Egg yolk, LDL


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How to Cite

Shahba, M.I., E.-S. R.I., and E.-A. A.I. “Freezability of Buffalo Semen by Using Tris Extender Supplemented With Different Concentrations of LDL”. Journal of Innovations in Pharmaceutical and Biological Sciences, vol. 3, no. 1, Jan. 2016, pp. 194-7,



Research Article