Freezability of buffalo semen by using tris extender supplemented with different concentrations of LDL

Abstract

The present study aimed at displaying the effect of LDL, as a cryopreservative, on the quality of the frozen-thawed buffalo semen. For buffalo semen extension, Tris-Citric acid-Fructose-Egg yolk extender was used as a control. Instead of Egg yolk, LDL fraction extracted from the Egg yolk would be added to the extender at6, 8, 10, and 12 % providing a concentration of 60 million sperm/ml. From the obtained results, the best semen quality was observed with the addition of 12% LDL to the frozen semen extender, as expressed by the highest sperm motility (32.50±1.50 %), sperm livability (72.00± 1.82 %), sperm cell membrane (69.10 ±1.84 %) and DNA (98.90 ±0.91 %) integrities as well as the lowest sperm abnormalities (09.10 ±1.48 %).  From the present study, the use of LDL at a concentration of 12% in buffalo frozen semen extender might be recommended to have better sperm motility, sperm livability and sperm abnormalities as well as sperm cell membrane and DNA integrities.