Assay for antimicrobial activity of mangosteen rind extracts

Abstract

Mangosteen rind that just used to be discarded, in a fact, it possesses a benefit to be developed as a phytopharmacal product. It contains quite a lot of compounds with pharmacological activity, such as anti-inflammatory, antihistamine, antibacterial, antifungal even for therapy or treatment of heart disease and HIV. The purpose of this study was to determine antibacterial activity of mangosteen rind extract (MRE) on growth of Escherichia coli. Effectiveness of antibacterial effect of the MRE, a conical flask containing 250 ml of sterilized agar was added with 0.5% bacterial suspension and then poured onto a petri dish and paper discs that had been dipped prior into MRE solution at various concentrations were placed onto the media and then incubated at 37ᵒC for 18-24h. Diameter of clear zones were performed around the paper discs were measured. The results showed that estimation amount of bacterial colonies of E. coli at dilution rate of 10^-6 was 9.7x10^-8 CFU/ ml. The results showed that the antibacterial activity of MRE at all levels of concentration effectively inhibited the growth of E. Coli. MRE showed more effectiveness and large areas of inhibitory against E. coli at a concentration of 10% which was equal to15 mm, while MIC at a concentration of 0.62% was equal to 5.75 mm. This suggests that increasing the concentration of MRE resulted in occurrence of the growth inhibition zones were greater. To learn more antibacterial activities of MRE, it must be tested against some gram positive bacterial strains and with different solvent extraction methods