TY - JOUR AU - S. Krause Ferrão, AU - G. Carlos, AU - J.C. Butze, AU - L. Mezzomo, AU - L. Noal Calil, AU - M. Anders Appel, AU - S. Bonato Luisci, AU - A. Mezzari, AU - R. Pereira Limberger, PY - 2021/07/01 Y2 - 2024/03/28 TI - Evaluation of protein mass Fingerprint-MS of clinical isolates of Candida spp. by MALDI-TOF MS JF - Journal of Innovations in Pharmaceutical and Biological Sciences JA - J Inn Pharm Bio Sci VL - 8 IS - 3 SE - Research Article DO - UR - https://jipbs.com/index.php/journal/article/view/410 SP - 24-30 AB - <p>Invasive fungal infections have high levels of mortality, especially in immunocompromised patients. Clinical isolates of <em>Candida</em> spp. are responsible for more than 50% of the researched examples. Matrix Assisted Laser Desorption Ionization Time of Flight (MALDI-TOF) offers fast and accurate identification of the microorganism, optimizing the beginning of antifungal therapy. In recent years, studies are being carried out on the applicability of MALDI-TOF in fungal identification. However, few data were found on fingerprint-MS in different species of <em>Candida</em>. The present study aims to evaluate a Protein Mass Fingerprint (PMF) of clinical isolates of <em>Candida</em> spp identified by MALDI-TOF MS. For this purpose, it was performed a multivariate analysis of the spectra. There was a consistent spectrum profile among <em>Candida</em> species between 3 and 15 KDa. The most intense peaks being between 3 and 8 KDa. It was noted the existence of characteristic signs between species. <em>C. albicans</em> shows two intense signs, the first at 4.3 and 4.4 KDa and the other at 5.1 and 5.2 KDa. The others species show an intense signal in 4.4 - 4.5 KDa.&nbsp; <em>C. lusitaniae</em> shows intense signals between 6.0 - 6.1 and 6.6 - 6.7 KDa, C<em>. guilhermondii</em> between 6.0 - 6.1 and 6.1 –6.2 KDa. <em>C. glabrata</em> an intense signal in 5.5 - 5.6 KDa and a less intense signal in 11.0-11.1 Kda. Thus, it is possible to outline a spectral profile of <em>Candida</em> spp. The characterization of the spectral profile can help in the critical evaluation of fingerprint-MS, assisting the analyst in the decision-making process and in the expansion of reference library.</p> ER -