TY - JOUR AU - Abdel-Rahman, Tahany M. AU - Neveen M. Khalil, AU - Abd El-Ghany, Mohamed N. AU - Enas Yosef, PY - 2019/01/05 Y2 - 2024/03/28 TI - Purification, characterization and medicinal application of tyrosinase extracted from Saccharomyces cerevisiae JF - Journal of Innovations in Pharmaceutical and Biological Sciences JA - J Inn Pharm Bio Sci VL - 6 IS - 1 SE - Research Article DO - UR - https://jipbs.com/index.php/journal/article/view/352 SP - 01-11 AB - <p>Extracellular tyrosinase extracted from <em>Saccharomyces cerevisiae</em> was purified, characterized and assayed as medicinal agent. The crude enzyme activity was 7.99 U/ml and specific activity was 0.84 U/mg. The complete purification protocol was done. The final result showed one peak of pure tyrosinase with activity of 1.08 U/ml, specific activity of 2.31 U/mg, purification fold of 31.25 and recovery of 22 %. The molecular mass of the enzyme was approximately 40 KDa. Characterization of the pure enzyme indicated that the optimum enzyme concentration was 4 mg protein/ml, however, optimum substrate [tyrosine] concentration was 2.2 mg/100 ml. The k<sub>m</sub> and V<sub>max</sub> values were 2.56 mg/ml and 20 U/ml, respectively. PH 9 proved to be the optimum value, while 35 °Cwas the optimum temperature. CuSO<sub>4</sub><em>&gt;</em>MgCl<sub>2</sub><em>&gt;</em>ZnSO<sub>4 </sub>enhanced the enzyme activity; however, HgSO<sub>4</sub> completely inhibited it. Assaying the enzyme inhibitors, declared that the metal chelating compound EDTA completely inhibited the enzyme activity which showed clearly that it is a metaloprotein. Medicinal applications of tyrosinase indicated that it exert weak antimicrobial activity against <em>Bacillus subtilis</em>, <em>Pseudomonas aeruginosa</em>, <em>E. coli</em> and <em>Staphylococus aureus</em>, but not have any antifungal activity. The enzyme proved to have antioxidant activity 50% of glutathione peroxidase. It also increased the viability of normal skin melanocyte cell line [MFB-4] before and after UV-irradiation indicated its protective and healing effect against UV-rays. The enzyme also reducing the viability of breast carcinoma cell line [MCF-7] after and before UV exposure declared that it may have anticancer activity</p> ER -