TY - JOUR AU - Amal A. Almousa, AU - El-Ghany, Mohamed N. Abd AU - Eman H. Ashour, PY - 2018/10/05 Y2 - 2024/03/29 TI - Citric Acid Fermentation by Aspergillus niger JF - Journal of Innovations in Pharmaceutical and Biological Sciences JA - J Inn Pharm Bio Sci VL - 5 IS - 4 SE - Research Article DO - UR - https://jipbs.com/index.php/journal/article/view/337 SP - 20-37 AB - <p>From twenty soil samples collected, ninety six isolates were identified as <em>Aspergillus niger</em>. All <em>A. niger</em> isolates understudying were varied in their capability to produce citric acid on Czapek-Dox broth medium. Based on the productivity of citric acid in shake flask cultures after 3 days, the highest local <em>A. niger</em> MH368137, was selected for further experiments with the reference strain, <em>A. niger</em> EMCC132. The optimal time incubation for the maximum citric acid production reached statistically 5 days of fermentation. Through series of experiments were designed on various physicochemical fermentation parameters to establish the optimal conditions for citric acid overproduction, it could be summarized as: optimum temperature, 28˚C; initial pH 6.5; inoculums size 3.0×10<sup>6</sup> spores/ml; shaking culture (100rpm); maltose as a carbon source 40 g/L; peptone as a nitrogen source 3 g/L; Sodium phosphate as phosphorus source; 2% of methanol as enhancer. Through these environmental and nutritional parameters, citric acid production was clearly improved and gave 14 times higher that reached to 13.33 mg/1ml comparing to the original yields 0.907 mg/ ml by <em>Aspergillus niger</em> MH368137 (FQW). When local agricultural wastes and byproducts (pineapple peel, sugarcane bagasse, potato peels; sugarcane molasses and dates molasses) utilized for citric acid production, local strain <em>A. niger</em> MH368137 (FQW) produced highest amount of citric acid, reached up to 256.94 mg/ ml when grew on sugarcane molasses as a sole carbon source</p> ER -