@article{Shahba, M.I._R.I._A.I._2016, title={Freezability of buffalo semen by using tris extender supplemented with different concentrations of LDL}, volume={3}, url={https://jipbs.com/index.php/journal/article/view/128}, abstractNote={<p>The present study aimed at displaying the effect of LDL, as a cryopreservative, on the quality of the frozen-thawed buffalo semen. For buffalo semen extension, Tris-Citric acid-Fructose-Egg yolk extender was used as a control. Instead of Egg yolk, LDL fraction extracted from the Egg yolk would be added to the extender at6, 8, 10, and 12 % providing a concentration of 60 million sperm/ml. From the obtained results, the best semen quality was observed with the addition of 12% LDL to the frozen semen extender, as expressed by the highest sperm motility (32.50±1.50 %), sperm livability (72.00± 1.82 %), sperm cell membrane (69.10 ±1.84 %) and DNA (98.90 ±0.91 %) integrities as well as the lowest sperm abnormalities (09.10 ±1.48 %).  From the present study, the use of LDL at a concentration of 12% in buffalo frozen semen extender might be recommended to have better sperm motility, sperm livability and sperm abnormalities as well as sperm cell membrane and DNA integrities.</p>}, number={1}, journal={Journal of Innovations in Pharmaceutical and Biological Sciences}, author={Shahba, M.I. and R.I., El-Sheshtawy, and A.I., El-Azab,}, year={2016}, month={Jan.}, pages={194–197} }