The effects of carbon and nitrogen sources on the growth of Isaria tenuipes (Peck.) Samson (DL0099) from Lam Dong, Vietnam

Abstract

Isaria tenuipes (Peck.) Samson is a mushroom with high medicinal value. This study evaluated the effects of carbon and nitrogen sources on the mycelium growth and fruiting body formation of I. tenuipes (Peck.) Samson (DL0099) from Lam Dong, Vietnam in in-vitro. The results have determined that glucose with a concentration of 20~40 g/L was suitable for the growth of DL0099. Organic nitrogen sources (peptone, malt extract, and yeast extract) were suitable for the growth of I. tenuipes (DL0099), in which peptone 10 g/L was the most suitable for the mycelium density and colony diameter. The formation of fungal fruiting bodies was best in the medium supplemented with malt extract 10 g/L. The results will be beneficial to mushroom growers in biomass production and fruit body formation of the Vietnam native I. tenuipes (Peck.) Samson