In vitro anti-Staphylococcus aureus activity of the methanol extract from Mitrephora thorelii Pierre’s branches and leaves in Lam Dong Province, Viet Nam

Abstract

We demonstrated that the methanol extract of Mitrephora thorelii Pierre's branches and leaves in Lam Dong, Vietnam is anti-Staphylococcus aureus strain (Sta1) of Phenikaa University, Hanoi, Viet Nam and Staphylococcus aureus ATCC 29213 strain (Sta2) from Thermo Fisher Scientific, USA. Invitro anti-Sta1 and Sta2 activity of the methanol extract of Mitrephora thorelii Pierre's branches and leaves were not significantly different. With a rise in extract content, the inhibitory zone's created diameter grew as well. The extract content of 100 mg/mL demonstrated the greatest antibacterial activity with a 25 mm inhibition zone diameter against Sta1 and 24 mm inhibition zone diameter against Sta2. Bacteria were not resistant to the extract at concentrations of 20; 80 and 100 mg/mL after 48, 72, and 96 hours. The lowest inhibitory concentration (MIC) was between 312.5 and 625 µg/mL. The minimum bactericidal concentration was found to be 1.25 mg/mL.