In Vitro Antioxidant Activity of Fresh and Shade Dried Tamarindus Indica Leaves using Different Solvents
DOI:
https://doi.org/10.56511/JIPBS.2021.v8.i04.pp22-27Abstract
Objective: The present study was aimed to ascertain the antioxidant status of different solvent leaf extracts of Tamarindus indica leaves and its potential for scavenging free radical species in the body and by the determination of their total phenolics content. Methods: The different solvent plant extract was screened for possible antioxidant activities by the determination of total phenolic content by using Folin – Ciocalten reagent method and 2, 2diphenyl 1 picrylhydrazyl (DPPH) free radical scavenging assay. Results: The results of this study revealed that, the percentage inhibition of different extracts of water (WE) and hydroalcoholic (HAE) of Tamarindus indica was concentration dependent with an effective concentration at fifty percent of 79.49 µg/ml and 82.96 µg/ml compared to that of standard with IC 50 of 77.08 µg/ml, but the petroleum ether (PEE) and chloroform (CE) extracts revealed a very poor antioxidant activity (significantly lower IC 50 values = 144.17µg/ml and 279.74µ/ml). Conclusion: The antioxidant screening results indicate that exciting DPPH radical scavenging activity was observed in water and hydroalcoholic leaf extract of Tamarindus indica in comparison with standard ascorbic acid. This suggests that T. indica extracts exhibit a great potential for antioxidant activity and may be useful for their future nutritional and medicinal developments.
Keywords:
Tamarindus indica, antioxidant activity, DPPH assay method, Folin Ciocalten methodDownloads
References
Rates SMK. Plant sources as drugs. Toxicon. Elsevier 2001; 39:603-613.
Ashish B, Swapnil G. Hypoglycemic effect of polyherbal formulation in alloxan induced diabetic rats. Pharmacology online 2011; 3:764-773.
Cacereves A, O. Cano, L. Agular and B. Samayoa. Plants used in Guatemala for the treatment of gastrointestinal disorder. Screening of 84 plants against enterobacteria. Journal of Ethnopharmacology 1990; 30(1):55-73.
Yamagishi S, Matsui T. Nitric oxide, a Janusfaced therapeutic target for diabetic microangiopathy – Friend or Foe. Pharmacol Res 2011; 64:187-194.
Dash B. Ayurvedic cures for common diseases. Hind Pocket Books 1991; 52:7.
Seth S. Herbs for Health and Beauty. India Book House publishers 1996; 19:10-12.
Farnsworth NR, Soejarto DD. Global importance of medicinal plants. Cambridge University Press, Cambridge, UK 1991; 2551.
Prohp TP, Onougbe IO, Onyebuagu PC, Omeni AA, Okoli RI, Obeto NP. Effects of Aqueous extract of Triplochiton scleroxylon on Red Blood Cells and Associated Parameters in Alloxan-Induced Diabetic Rabbits. Pakistan Journal of Nutrition 2000; 5(5):425-33.
Patel DK, Kumar R, Laloo D, Hemalatha S. Natural medicines from plant source used for therapy of diabetes mellitus: An overview of its pharmacological aspects. Asian Pacific Journal of Tropical Biomedicine 2012; 239-311.
Muthu SE, Nandakumar S, Roa UA. The effect of methanolic extract of Tamarindus indica on the growth of clinical isolates of Burkholderiapseudomallei. Indian J Med Res 2005; 122:525-528.
Samina KK, Shaikh W, Shahzadi S, Kazi TG, Usmanghani K, Kabir A. Chemical constituents of Tamarindus indica. Medicinal plant in Sindh. Pak J Bot 2008; 40:2553-2559.
Lewis GB, Schrire B, Mackinder B, Lock M. Legumes of the World. Royal Botanic Gardens, Kew. Richmond, UK 2005; 2532.
Komutarin T, Azadi S, Butterworth L, Keil D, Chitsomboon B, Suttajit M, Meade BJ. Extract of the seed coat of Tamarindus indica inhibits nitric oxide production by murine macrophages in vitro and in vivo. Food and Chemical Toxicology 2004; 42:649-658.
Maiti R, Jana D, Das UK, Ghosh D. Antidiabetic effect of aqueous extract of seed of Tamarindus indica in streptozotocininduced diabetic rats, J of Ethnopharmacol 2004; 92:85 – 91.
Jain R, Jain S, Sharma A, Hideyuki I, Hatano T. Isolation of (+)-pinitol and other constituents from the root bark of Tamarindus indica Linn. J Nat Med 2007; 6:355 – 356.
Ashish Saraf, Sadaf Quereshi, Kavita Sharma. Antimicrobial activity if lantana camara L. Journal of Experimental sciences 2011; 2(10):50 – 54.
Sarita Manandhar, ShisirLuitel, Raj Kumar Dahal. In vitro Antimicrobial activity of some medicinal plants against human pathogenic bacteria. Journal of tropical medicine 2019;1 – 5.
Vorro ET, Lynn RB, James ER. Pharmacognosy. Lea and Febiger, Philadelphia 1981; 6:235-254.
Manzocco, L. Anese M, Nicoli M C. Antioxidant properties of tea extracts as affected by processing. Lebensmittel Wissenschaft Und Technologie 1998; 31(7-8):694-698.
Kunchandy, E, Rao, MNA. Oxygen radical scavenging activity of curcumin, Int. J. Pharm 1990; 58:237-240.
Md. Mahbubur Rahman, Md. Badrul Islam, Mohitosh Biswas. In vitro antioxidant and free radical scavenging activity of different parts of Tabebuia pallida growing in Bangladesh. BMC Res Notes 2015; 8:621.
Maxwell AfariGyamfi, MasatoYonamine, Yoko Aniya. Free radical scavenging action of medicinal herbs from Ghana Thonningiasanguinea on experimentally – induced liver injuries. General pharmacology 1998; 32:661-667.
Badakhshan Mahdi Pour, Subramanion L Jothy. Antioxidant activity of methanol extract of different parts of Lantana camara. Asian Pac J Trop Biomed 2012; 2(12):960-965.
Md. Nur Alam, Nusrat Jahan Bristi. Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharmaceutical Journal 2013; 21:143-152.
C LAsadu, C A Anosike, PN Uzoegwu. In vitro antioxidant activity of methanol extract of Lantana camara leaves. Global veterinaria 2015; 14(4):595-602.
Published
How to Cite
Issue
Section
Copyright (c) 2021 Mahbooba M K, Vandana K, Lal Prasanth M L, Shibu Prasanth C R
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Journal of Innovations in Pharmaceutical and Biological Sciences is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. Based on a work at