Combination effect of ethylacetate extract leaves of Moringa oleifera L. and Doxorubicin against MCF-7 cell lines
Objective: This study aim is to determine the effect of ethylacetate extract (EAE) of Moringa oleifera L. leaf and its combination with doxorubicin on cytotoxic effect, combination effect, cell cycle, apoptosis on MCF-7 cell lines. Methods: In vitro cytotoxic assays was determined by MTT (Microculture Tetrazolium Tehnique) assay, combined test was analysis with Compusyn software version 1, cell cycle inhibition and apoptosis were determined with Flowcytometry. Result: Cytotoxic activity of EAE with IC50 value 149.29 mg/mL, combination test of very strong synergistic at 1/8 IC50 EAE-1/4 IC50 doxorubicin concentration. The combination were caused cell accumulation in the G0/G1 phase, increased early and late apoptosis on MCF-7 cell lines. Conclusions: Based on the results combination giving a very strong synergistic effect. Therefore more research is needed on the mechanism
Keywords:Combination effect, Moringa oleifera L., MCF-7, Doxorubicin, Ethylacetate
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Copyright (c) 2018 Sherly H. Hutagaol, Rosidah, Masfria, Denny Satria
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