Combination effect of ethylacetate extract leaves of Moringa oleifera L. and Doxorubicin against MCF-7 cell lines

Authors

  • Sherly H. Hutagaol Department of Pharmacology, Faculty of Pharmacy, University of Sumatera Utara, Medan, Indonesia
  • Rosidah Department of Pharmacology, Faculty of Pharmacy, University of Sumatera Utara, Medan, Indonesia
  • Masfria Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Sumatera Utara, Medan, Indonesia
  • Denny Satria Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Sumatera Utara, Medan, Indonesia

Abstract

Objective: This study aim is to determine the effect of ethylacetate extract (EAE) of Moringa oleifera L. leaf and its combination with doxorubicin on cytotoxic effect, combination effect, cell cycle, apoptosis on MCF-7 cell lines. Methods: In vitro cytotoxic assays was determined by MTT (Microculture Tetrazolium Tehnique) assay, combined test was analysis with Compusyn software version 1, cell cycle inhibition and apoptosis were determined with Flowcytometry. Result: Cytotoxic activity of EAE with IC50 value 149.29 mg/mL, combination test of very strong synergistic at 1/8 IC50 EAE-1/4 IC50 doxorubicin concentration. The combination were caused cell accumulation in the G0/G1 phase, increased early and late apoptosis on MCF-7 cell lines. Conclusions: Based on the results combination giving a very strong synergistic effect. Therefore more research is needed on the mechanism

Keywords:

Combination effect, Moringa oleifera L., MCF-7, Doxorubicin, Ethylacetate

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Published

05-07-2018
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How to Cite

Sherly H. Hutagaol, Rosidah, Masfria, and Denny Satria. “Combination Effect of Ethylacetate Extract Leaves of Moringa Oleifera L. And Doxorubicin Against MCF-7 Cell Lines”. Journal of Innovations in Pharmaceutical and Biological Sciences, vol. 5, no. 3, July 2018, pp. 42-46, https://jipbs.com/index.php/journal/article/view/329.

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Research Article