Characterization of lipase produced by Aneurinibacillus aneurinilyticus strain LP-II isolated from soil of oil mill

Abstract

Biotechnological important lipase producing bacterium strain LP-II was isolated from local oil mill soil at Gangakhed (India). Lipase is an important industrially employed enzyme and used in trans-esterification of algae oil to biodiesel. LP-II is Gram positive log rods have showed growth at 40^oC and pH 6.7. LP-II has utilized dextrose and fructose as carbon source and produced acid in the medium. LP-II lipase produced lipases 1.5 U/mL at 40⁰C and 8.0 PH after 48 hours of incubation at 150 rpm. In addition, LP-II have showed positive result for gelatinase, caseinase oxidase and catalase tests at its optimum temperature and pH. Strain LP-II was identified as Aneurinibacillus aneurinilyticus using morphological, and biochemical methods. Identification made in former step was confirmed by 16SrRNAgene sequencing method as Aneurinibacillus aneurinilyticus strain LP-II (Accession no.: MF696161). LP-II lipases have an ability to work at moderate temperature and slightly high pH. Thus, lipase produced by LP-II strain may find application in various industrial processes and household chemicals